Tampere Imaging Facility (TIF)
Introduction
Tampere Imaging Facility (TIF) - Light Microscopy Core
The light microscopy facility offers state-of-the-art equipment for mesoscopic and high resolution bioimaging of tissues, small model organisms, biomaterials, tissue engineering products and advanced cell cultures both in 2D and 3D thus offering services for both life scientists and biomedical engineers.
Tampere Imaging Facility (TIF) is a light microscopy core unit located in the Arvo building on the Kauppi campus of the Tampere University. It offers state-of-the-art imaging solutions for life and material scientists. TIF includes several microscope systems for bioimaging of living or fixed specimens: spinning disk and laser scanning confocal microscopes, super-resolution structured illumination microscope, long timelapse imaging systems and widefield fluorescence microscopes. TIF also has data processing and analysis stations available with deconvolution, visualization and image quantification possibilities.
TIF provides training for users, organizes imaging related courses and actively participates teaching at the Tampere University. Users have the possibility to plan and conduct the experiments and data analysis together with the core personnel.
Booking system
Agendo booking system for the microscopes:
https://tuni.agendo.science
ACKNOWLEDGEMENT
All the users of the Tampere Imaging Facility (TIF) services are obligated to acknowledge the facility and Biocenter Finland in publications:
“The authors acknowledge the Biocenter Finland (BF) and Tampere Imaging Facility (TIF) for the service.”
Superresolution Microscopes
N-SIM (Nikon Structured Illumination Microscopy) super-resolution system for fixed and living samples. N-SIM doubles the resolution of conventional optical microscopes; resolution will depend on laser wavelength and imaging mode. This system is combined with Nikon A1R+ Laser Scanning Confocal Microscope.
Features:
Nikon Eclipse Ti2-E inverted microscope
Motorized XY stage with encoders
MCL Piezo Stage Insert and Z drive
Large incubator with heating
Stage-top incubator with heating and CO2
Perfect Focus System®
Super-Resolution:
Lateral (XY): ~100-130 nm (dependent on wavelength and optics)
Axial (Z): ~300 nm (dependent on wavelength and optics)
3D Axial Range: up to 20 μm (~8 µm oil, ~20 µm water)
Resolution depends on dye properties
Objectives:
Nikon Plan Apo VC 20x DIC N2, N.A. 0.75, WD 1.00 mm (Air)
Nikon Apo LWD 40x WI λS DIC N2, N.A. 1.15, WD 0.61 mm (Water)
Nikon CFI Plan Apo IR SR 60x WI, N.A. 1.27, WD 0.18 mm (Water)
Nikon Apo 60x Oil λS DIC N2, N.A. 1.40, WD 0.13 mm (Oil)
Nikon CFI Apo SR TIRF 100X Oil, N.A. 1.49, WD 0.12 mm (Oil)
Nikon SR HP Plan Apo 100x Sil, N.A. 1.35, WD 0.30 mm (Silicone)
Lasers:
Solid state 405 nm, 488 nm, 561 nm, 640 nm lasers built-in AOTF (Acousto-Optical Tunable Filter)
N-SIM Camera and Filters:
EM CCD camera iXon3 DU-897E (Andor Technology Ltd.)
N-SIM CH Filter Cubes for 405, 488, 561, 640
Software:
NIS-Elements AR with software module NIS-A N-SIM Analysis
Current Version: NIS-Elements AR 5.21.03 (November 2021)
Confocal Microscopes
Laser scanning confocal imaging system for fixed and living samples. Incubation and hardware based autofocus (Perfect Focus System®). In addition to galvano scanner, equipped with a resonant scanner for capturing confocal images at high-speed.
Features:
Nikon Eclipse Ti2-E inverted microscope
Motorized XY stage with encoders
MCL Piezo Stage Insert and Z drive
Large incubator with heating
Stage-top incubator with heating and CO2
Perfect Focus System®
A1R+ equipped with both fast resonant and high-resolution galvano scanners
FRAP/FLIP/Photoactivation possibility
Holders for sliders, dishes, and multiwell plates
Objectives:
Nikon Plan Apo VC 20x DIC N2, N.A. 0.75, WD 1.00 mm (Air)
Nikon Apo LWD 40x WI λS DIC N2, N.A. 1.15, WD 0.61 mm (Water)
Nikon CFI Plan Apo IR SR 60x WI, N.A. 1.27, WD 0.18 mm (Water)
Nikon Apo 60x Oil λS DIC N2, N.A. 1.40, WD 0.13 mm (Oil)
Nikon CFI Apo SR TIRF 100X Oil, N.A. 1.49, WD 0.12 mm (Oil)
Nikon SR HP Plan Apo 100x Sil, N.A. 1.35, WD 0.30 mm (Silicone)
Lasers:
Solid state 405 nm, 488 nm, 561 nm, 640 nm lasers built-in AOTF (Acousto-Optical Tunable Filter)
Detectors and Filters:
A1-DUG GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 normal PMTs
Emission filters: 450/50, 482/35, 525/50, 540/30, 595/50, 700/75
Transmitted Light:
DIC
Software:
NIS-Elements AR
(with software module NIS-A N-SIM Analysis)
Current Version: NIS-Elements AR 5.21.03 (November 2021)
Nikon Eclipse FN1 is combined widefield and spinning disk confocal microscope for fixed and living specimens. It is equipped with micromanipulation and patch clamp possibility.
Features:
Dipping objectives
16 LED fluorescence light source
DIC, IR-DIC
Reflection imaging
Extra zoom unit (0.6x-2.0x)
Objective and sample stage heaters
Crest Optics X-LightTM V2TP confocal imager
Objectives:
Nikon Plan Fluor 10x/0.3, WD 16.0 mm (Air)
Nikon Plan Fluor ELWD 20x/0.45, WD 7.4 mm (Air)
Nikon CFI Apo LWD 25x/1.1, WD 2.0 mm (Water) (dipping)
Nikon CFI Apo NIR 40x/0.8, WD 3.5 mm (Water) (dipping)
Nikon Plan Fluor 40x/1.3, WD 0.24 mm (Oil)
Nikon CFI Apo NIR 60x/1.0, WD 2.8 mm (Water) (dipping)
Nikon CFI Plan 100x/1.1, WD 2.5 mm (Water) (dipping)
Light Sources:
CoolLED pE-4000
Lumencor SPECTRA X Light Engine
Cameras:
Hamamatsu Orca-Flash4.0 V2 CMOS camera
Andor iXon 888 EMCCD camera
Transmitted Light:
Brightfield
DIC
IR-DIC
Software:
NIS-Elements AR
Current Version: NIS-Elements AR 5.11.00 (September 2018)
Laser scanning confocal microscope for spectral imaging of fixed samples. User friendly design, simultaneous detection of three color signals. Separation of the fluorescence signals by selective laser excitation coupled to efficient splitting of the emission using the variable secondary dichroic (VSD) beamsplitter.
Features:
Zeiss Axio Observer.Z1 inverted microscope
Motorized Z-drive operation
Motorized stage for multipoint imaging
Mounting frame for specimen sliders 76×26 mm
Objectives:
Zeiss EC Plan-Neofluar 5x/0.16, WD 18.5 mm (Air)
Zeiss EC Plan-Neofluar 10x/0.30, WD 5.2 mm (Air)
Zeiss EC Plan-Neofluar 20x/0.50, WD 2.0 mm (Air)
Zeiss LD LCI Plan-Apochromat 25x/0.80, WD 0.57 mm (Water, Silicone oil, Glycerine and Oil)
Zeiss EC Plan-Neofluar 40x/0.75, WD 0.71 mm (Air)
Zeiss Plan-Apochromat 63x/1.40, WD 0.19 mm (Oil)
Lasers:
Solid state diode lasers: 405 nm, 488 nm, 561 nm, and 640 nm
Main Beam Splitters: MBS 405/488/561/640
Detectors:
3-channel gallium arsenide phosphide photomultiplier tube detector (GaAsP-PMT)
Transmitted light photomultiplier tube detector (T-PMT)
Light Sources and Filters (eyepieces):
CoolLED pE-300lite white light LED illuminator
100W halogen illuminator
Zeiss Filter set 49: BP 335-383, FT 395, BP 420-470
Zeiss Filter set 60: HE CFP/YFP/HCRed
BP 432-457, BS 460, BP 469-491
BP 502-517, BS 520, BP 530-560
BP 574-601, BS 605, LP 615
Zeiss Filter set 15: BP 540-552, BS 580, LP 590
Transmitted Light:
Brightfield
DIC (Plan-Apochromat 25x/0.80, Plan-Apochromat 63x/1.40)
Software:
ZEN Blue 3.6
Current Version: ZEN Blue 3.6.9 (May 2023)
Laser scanning confocal microscope for fixed and living samples. Equipped with spectral detector (no conventional filters) using sensitive GaAsP-technology. Fluorescence Correlation Spectroscopy (FCS), FRAP/FLIP/Photoactivation possibility.
Features:
Zeiss Cell Observer.Z1 inverted microscope
Large incubator with heating
Stage-top incubator with heating and CO2
Motorized stage for multipoint imaging
Fluorescence Correlation Spectroscopy (FCS) possibility
Holders for sliders, dishes, and multiwell plates
Objectives:
Zeiss Plan-Apochromat 10x/0.45, WD 2.00 mm (Air)
Zeiss LD LCI Plan-Apochromat 25x/0.80, WD 0.57 mm (Water, Silicone oil, Glycerine and Oil)
Zeiss LD C-Apochromat 40x/1.10, WD 0.62 mm (Water)
Zeiss Plan-Apochromat 40x/1.40, WD 0.24 mm (Oil)
Zeiss C-Apochromat 63x/1.20, WD 0.28 mm (Water)
Zeiss Plan-Apochromat 63x/1.40, WD 0.19 mm (Oil)
Lasers:
Multiline Argon laser: 458 nm, 488 nm, and 514 nm
Diode laser 405 nm
Diode-Pumped Solid-State (DPSS) 561 nm
HeNe-lasers 594 nm, and 633 nm
(No longer available:
InTune-tunable pulsed laser 490 nm – 645 nm)
Main Beam Splitters (Visible Light):
Plate
MBS 458
MBS 458/514
MBS 458/561
MBS 488
MBS 488/561
MBS 488/633
MBS 488/561/633
MBS 488/594
MBS T80/R20
Secondary Beam Splitters (Invisible Light):
Plate
f-MBS 405/490c/640c
f-MBS 405/505c
f-MBS 405/520c
f-MBS 405/535c
f-MBS 405/550c
f-MBS 405/565c
f-MBS 405/580c
f-MBS 405/595c
f-MBS 405/610c
f-MBS 405/625c
MBS T80/R20
Detectors:
32-channel QUASAR detector: gallium arsenide phosphide photomultiplier tube detector (GaAsP-PMT)
Two conventional multialkali photomultiplier tube detectors (PMTs)
Transmitted light photomultiplier tube detector (T-PMT)
Light Sources and Filters (eyepieces):
CoolLED pE-300lite white light LED illuminator
100W halogen illuminator
Zeiss Filter Set 49: EX G 365, FT 395, EM BP 445/50
Zeiss Filter Set 38: EX BP 470/40, FT 495, EM BP 525/50
Zeiss Filter Set 43: EX BP 545/25, FT 570, EM BP 605/70
Transmitted Light:
Brightfield
DIC
Software:
ZEN Black 2.3 SP1
Current Version: ZEN Black 2.3 SP1 FP3 (July 2020)
Timelapse Imaging Systems
Cell-IQ® is automated cell culture and analysis system for kinetic cell studies. Operating Cell-IQ is simple requiring minimum set up. LED illumination for both phase and fluorescence imaging reducing external cell effects such as photo-toxicity and photobleaching.
Features:
The system comprises incubator with heating
Independent gas feeds up to two multiwell plate positions
Compatible with 6-, 12-, 24-, 48-, and 96-well microplates
Autofocus and Z-stack possibility
Objectives:
Nikon Plan Fluor 4x/0.13, WD 16.5 mm (Air)
Nikon Plan Fluor 10x/0.30, WD 15.2 mm (Air)
Nikon S Plan Fluor ELWD 20x/0.45, WD 8.2−6.9 mm (Air)
Light sources and Filters:
PE Excitation system with LED light source (470 nm, 550 nm, 660 nm)
Semrock Filter set GFP/488 (LF488): EX BP 482/9, EM LP 505, DM RB 471−491 (longpass)
Semrock Filter set Cy3-C/568 (LF561/LP-A-000): EX BP 561/7, EM LP 585, DM RB 554−568 (longpass)
Semrock Filter set Cy5-C/680 (Cy5.5-B-000): EX BP 655/20, EM BP 716/20, DM RB 350−676 (bandpass)
Camera:
Retiga EXi 1392 CCD monochrome camera
Transmitted Light:
Phase Contrast
Software:
Cell-IQ ImagenTM
Image Processing Software:
Cell-IQ AnalyserTM
Widefield Fluorescence Microscopes
The AZ100 is a multi-purpose zoom microscope system for fixed and living samples. Smooth zooming mechanism and a triple nosepiece enables continuous switching of magnifications, extending from macro to micro observation of the same specimen.
Features:
Nikon AZ100 Multizoom upright microscope
Built-in 8x zoom optics, which provide from 1x to 8x magnification
Stand section offers an 85 mm and the stage section a 10 mm stroke; even tall samples can be observed
Objectives:
Nikon AZ-Plan Apo 1x/0.10, WD 35.0 mm (Air)
Nikon AZ-Plan Fluor 2x/0.20, WD 45.0 mm (Air)
Nikon AZ-Plan Fluor 5x/0.50, WD 15.0 mm (Air)
Light Sources and Filters:
Nikon Intensilight C-HGFI
Nikon C-FLED LED Light Source
Semrock FF01-520/35-25: 520/35 nm BrightLine® single-band bandpass filter
Semrock FF01-543/22-25: 543/22 nm BrightLine® single-band bandpass filter
BSS10R UV Fused Silica Broadband Plate Beamsplitter for reflected light
Camera:
The Imaging Source DFK 33UX250 CMOS color camera
Transmitted Light:
Brightfield
Software:
NIS-Elements D
Current Version: NIS-Elements D 5.02.00 (May 2018)
Widefield fluorescent microscope for fixed samples. User-friendly system specially when long working distance is needed; operating with multiwell plastic plates as well as with glass objective slides.
Features:
Olympus IX51 inverted microscope
Stage holder for multiwell plates
Specimen holder for slides 76×26 mm
Objectives:
Olympus UPlanFL N 4x/0.13, WD 17.0 mm (Air)
Olympus CPlanFL N 10x/0.30, WD 9.5 mm (Air)
Olympus LUCPlan FL N 20x/0.45, WD 6.6−7.8 mm (Air)
Olympus LUCPlan FL N 40x/0.60, WD 3.0−4.2 mm (Air)
Olympus UPlanSApo 40x/0.95, WD 0.18-0.2 mm (Air)
Light Sources and Filters:
Olympus U-LGPS LED Light Source
100 W halogen illumination
Olympus U-MNU2: EX 360-370, EM 420, DM 400 (longpass)
Olympus U-MNUA2: EX 360-370, EM 420-460, DM 400 (bandpass)
Olympus U-MNIBA3: EX 470-495, EM 510-550, DM 505 (bandpass)
Olympus U-MNB2: EX 470-490, EM 520, DM 500 (longpass)
Olympus U-MNIGA3: EX 540-550, EM 575-625, DM 570 (bandpass)
Olympus U-NA49006: EX 590-650, EM 665-735, DM 660 (bandpass)
Camera:
Hamamatsu Orca Flash4.0LT+ sCMOS camera
Transmitted Light:
Brightfield
Phase Contrast
Software:
Olympus cellSens Dimension 2
Current Version: cellSens Dimension 2.3 (February 2021)
Widefield fluorescence microscope for fixed samples. Compact system with a large range of features. Equipped high-quality color camera with the large field of view and number of pixels (2752 horizontal pixels × 2208 vertical pixels). Axiocam 506 color offers a dynamic range of 1:2500 for optimized color reproduction.
Features:
Zeiss Axio Scope.A1 upright microscope
Specimen holder for sliders 76×26 mm
Objectives:
Zeiss N-Achroplan 5x/0.15, WD 12.0 mm (Air)
Zeiss Plan-Apochromat Ph1 10x/0.45, WD 2.1 mm (Air)
Zeiss LD Plan-Apochromat Ph2 20x/0.80, WD 0.55 mm Air)
Zeiss LD Plan-Apochromat Corr Ph3 40x/0.95, WD 0.25 mm (Air)
Zeiss Plan-Neofluar 63x/1.25, WD 0.10 mm (Oil)
Zeiss Plan-Neofluar 100x/1.30, WD 0.20 mm (Oil)
Light Sources and Filters:
Zeiss HXP 200C Fluorescence Illuminator
100 W halogen illumination
Zeiss Filter set 49: EX G 365, BS FT 395, EM BP 445/50
Zeiss Filter set 38: EX BP 470/40, BS FT 495, EM BP 525/50
Zeiss Filter set 20: EX BP 546/12, BS FT 560, EM BP 575-640
Zeiss Filter set 50: EX BP 640/30, BS FT 660, EM BP 690/50
Camera:
Zeiss Axiocam 506 color
Transmitted Light:
Brightfield
Darkfield
Phase Contrast
Software:
ZEN Blue 3.0
Current Version: ZEN Blue 3.0 (June 2020)
Image Processing
Tampere Imaging Facility offers analysis computer for image processing in dedicated office. Specially meant for deconvolution with Scientific Volume Imaging Huygens Essential and for 3D/4D visualization and analysis with Oxford Instruments Imaris.
Image Analysis Deconvolution and Imaris:
Lenovo P920 ThinkStation
Processor: Intel(R) Xeon(R) Silver 4210R CPU 2.40GHz 2.39 GHz (2 processors)
Installed RAM: 256 GB
Graphics card: NVIDIA MSI GeForce RTX 3090 SUPRIM X 24GB
Scientific Volume Imaging Huygens Essential:
Huygens Essential is tailored for deconvolution and processing of microscopy images. Its wizard-driven user interface guides you through the process of deconvolving microscopy images.
Current Version: Huygens 23.04 (May 2023)
Oxford Instruments Imaris:
Imaris Single Full includes functionalities for multiple image analysis needs. Visualization of 3/4D microscopy datasets with automated Spots and Surfaces detection and visualization, smart detection of complex objects, tracing of neurons, blood vessels (no lumen) or other filamentous structures, tracking including cell division detection, batch analysis and a wide range of customized analysis powered by MatLab or Python.
Current Version: Imaris 10.0.1 (May 2023)
NIS-Elements Advanced Research (AR) Microscope Imaging Software:
Nikon’s powerful software for visualization, data management and analysis. Offers many image processing features and handles large data sets.
Current Version: NIS-Elements AR 5.02.03 (September 2022)
Adobe Creative Cloud:
Including Photoshop, Illustrator, InDesign, Premiere Pro, and more. Sign in with your own Adobe ID and password.
Other available softwares:
Blender
CellProfiler
Fiji-ImageJ
MATLAB
NDP.view 2
NIS-Elements Viewer
LAS X Core Offline
ZEN lite
Prices
| SYSTEM RESERVATION: COMPUTERS | PRICE/HOUR |
| Image Analysis computer for deconvolution and other image processing (Agendo name: Image Analysis Deconvolution and Imaris) |
1,50 € |
| SYSTEM RESERVATION: CONFOCAL MICROSCOPES | PRICE/HOUR |
| Nikon A1R+ laser scanning confocal microscope (Agendo name: Nikon A1R with N-SIM) |
22,00 € |
| Nikon Eclipse FN1 upright spinning disk confocal microscope (Agendo name: Nikon FN1 Upright Fluorescence Microscope) |
8,00 € |
| Zeiss LSM 800 laser scanning confocal microscope (Agendo name: Zeiss LSM 800 LSCM) |
15,00 € |
| Zeiss LSM 780 laser scanning confocal microscope (Agendo name: Zeiss LSM 780 LSCM) |
20,00 € |
| SYSTEM RESERVATION: SUPER-RESOLUTION MICROSCOPES | PRICE/HOUR |
| Nikon N-SIM super-resolution microscope system (Agendo name: Nikon A1R with N-SIM) |
22,00 € |
| SYSTEM RESERVATION: TIMELAPSE IMAGING | PRICE/HOUR |
| CM Technologies Cell-IQ® automated imaging and analysis system (Agendo name: CM Technologies Cell-IQ slot 1) |
1,50 € |
| SYSTEM RESERVATION: WIDEFIELD FLUORESCENCE MICROSCOPES | PRICE/HOUR |
| Olympus IX51 inverted fluorescence microscope (Agendo name: Olympus IX51 Fluorescence Microscope) |
1,00 € |
| Zeiss Axio Scope.A1 upright fluorescence microscope (Agendo name: Zeiss Axio Scope.A1 Fluorescence Microscope) |
1,00 € |
| Nikon AZ100 multizoom upright fluorescence macroscope (Agendo name: Nikon AZ100 Fluorescence Macroscope) |
2,00 € |
Pricing is valid for user groups from the Tampere University. Other academic or non-academic users are asked to inquire specific pricing.
Prices above are applicable from Monday to Friday 8 am to 4 pm. Discount of 50 % is applied for reservations on night time between 4 pm to 8 am and on weekends.
Tampere Imaging Facility reserves the right to price adjustments.
Tampere Imaging Facility Publications
2023
Hyväri L, Vanhatupa S, Ojansivu M, Kelloniemi M, Pakarinen TK, Hupa L, Miettinen S.
Heat Shock Protein 27 Is Involved in the Bioactive Glass Induced Osteogenic Response of Human Mesenchymal Stem Cells.
Cells. 2023 Jan 5;12(2):224. doi: 10.3390/cells12020224.
PMID: 36672159
Alexanova A, Raitoharju E, Valtonen J, Aalto-Setälä K, Viiri LE.
Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism.
Sci Rep. 2023 Jan 30;13(1):1706. doi: 10.1038/s41598-023-28981-7.
PMID: 36717592
Hellinen L, Hongisto H, Ramsay E, Kaarniranta K, Vellonen KS, Skottman H, Ruponen M.
Comparison of barrier properties of outer blood-retinal barrier models - Human stem cell-based models as a novel tool for ocular drug discovery.
Eur J Pharm Biopharm. 2023 Mar;184:181-188. doi: 10.1016/j.ejpb.2023.01.026.
PMID: 36740104
Karema-Jokinen V, Koskela A, Hytti M, Hongisto H, Viheriälä T, Liukkonen M, Torsti T, Skottman H, Kauppinen A, Nymark S, Kaarniranta K.
Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients.
J Biol Chem. 2023 May 1:104770. doi: 10.1016/j.jbc.2023.104770.
PMID: 37137441
Mahmoud M, Juntunen M, Adnan A, Kummola L, Junttila IS, Kelloniemi M, Tyrväinen T, Huhtala H, Abd El Fattah AI, Amr K, El Erian AM, Patrikoski M, Miettinen S.
Immunomodulatory Functions of Adipose Mesenchymal Stromal/Stem Cell Derived From Donors With Type 2 Diabetes and Obesity on CD4 T Cells.
Stem Cells. 2023 May 15;41(5):505-519. doi: 10.1093/stmcls/sxad021.
PMID: 36945068
Hammarén MM, Luukinen H, Sillanpää A, Remans K, Lapouge K, Custódio T, Löw C, Myllymäki H, Montonen T, Seeger M, Robertson J, Nyman TA, Savijoki K, Parikka M.
In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies.
mSystems. 2023 May 15:e0107322. doi: 10.1128/msystems.01073-22.
PMID: 37184670
Sartoneva R, Lyyra I, Juusela M, Sharma V, Huhtala H, Massera J, Kellomäki M, Miettinen S.
In vitro biocompatibility of polylactide and polybutylene succinate blends for urethral tissue engineering.
J Biomed Mater Res B Appl Biomater. 2023 May 18. doi: 10.1002/jbm.b.35268.
PMID: 37199480
2022
Saari J, Siddique F, Korpela S, Mäntylä E, Ihalainen TO, Kaukinen K, Aalto-Setälä K, Lindfors K, Juuti-Uusitalo K.
Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells.
Int J Mol Sci. 2022 Jan 24;23(3):1312. doi: 10.3390/ijms23031312.
PMID: 35163236
Viheriälä T, Hongisto H, Sorvari J, Skottman H, Nymark S, Ilmarinen T.
Cell maturation influences the ability of hESC-RPE to tolerate cellular stress.
Stem Cell Res Ther. 2022 Jan 24;13(1):30. doi: 10.1186/s13287-022-02712-7.
PMID: 35073969
Mykuliak A, Yrjänäinen A, Mäki AJ, Gebraad A, Lampela E, Kääriäinen M, Pakarinen TK, Kallio P, Miettinen S, Vuorenpää H.
Vasculogenic Potency of Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells Results in Differing Vascular Network Phenotypes in a Microfluidic Chip.
Front Bioeng Biotechnol. 2022 Feb 8;10:764237. doi: 10.3389/fbioe.2022.764237.
PMID: 35211462
Häkli M, Jäntti S, Joki T, Sukki L, Tornberg K, Aalto-Setälä K, Kallio P, Pekkanen-Mattila M, Narkilahti S.
Human Neurons Form Axon-Mediated Functional Connections with Human Cardiomyocytes in Compartmentalized Microfluidic Chip.
Int J Mol Sci. 2022 Mar 15;23(6):3148. doi: 10.3390/ijms23063148.
PMID: 35328569
Gaballah M, Penttinen K, Kreutzer J, Mäki AJ, Kallio P, Aalto-Setälä K.
Cardiac Ischemia On-a-Chip: Antiarrhythmic Effect of Levosimendan on Ischemic Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Cells. 2022 Mar 19;11(6):1045. doi: 10.3390/cells11061045.
PMID: 35326497
Fadjukov J, Wienbar S, Hakanen S, Aho V, Vihinen-Ranta M, Ihalainen TO, Schwartz GW, Nymark S.
Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium.
J Gen Physiol. 2022 Apr 4;154(4):e202112916. doi: 10.1085/jgp.202112916.
PMID: 35275193
Mzezewa R, Lotila J, Kiiski H, Vinogradov A, Kapucu FE, Peltola J, Hagman S, Narkilahti S.
A kainic acid-induced seizure model in human pluripotent stem cell-derived cortical neurons for studying the role of IL-6 in the functional activity.
Stem Cell Res. 2022 Apr;60:102665. doi: 10.1016/j.scr.2022.102665.
PMID: 35091307
Gebraad A, Ohlsbom R, Miettinen JJ, Emeh P, Pakarinen TK, Manninen M, Eskelinen A, Kuismanen K, Slipicevic A, Lehmann F, Nupponen NN, Heckman CA, Miettinen S.
Growth Response and Differentiation of Bone Marrow-Derived Mesenchymal Stem/Stromal Cells in the Presence of Novel Multiple Myeloma Drug Melflufen.
Cells. 2022 May 7;11(9):1574. doi: 10.3390/cells11091574.
PMID: 35563880
Peussa H, Kreutzer J, Mäntylä E, Mäki AJ, Nymark S, Kallio P, Ihalainen TO.
Pneumatic equiaxial compression device for mechanical manipulation of epithelial cell packing and physiology.
PLoS One. 2022 Jun 3;17(6):e0268570. doi: 10.1371/journal.pone.0268570.
PMID: 35657824
Samanta S, Ylä-Outinen L, Rangasami VK, Narkilahti S, Oommen OP.
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain mimetic 3D scaffold.
Acta Biomater. 2022 Jul 15;147:439. doi: 10.1016/j.actbio.2022.05.025.
PMID: 35597769
Korkka I, Skottman H, Nymark S.
Heterogeneity of Potassium Channels in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium.
Stem Cells Transl Med. 2022 Jul 20;11(7):753-766. doi: 10.1093/stcltm/szac029.
PMID: 35639962
Azizi L, Varela L, Turkki P, Mykuliak VV, Korpela S, Ihalainen TO, Church J, Hytönen VP, Goult BT.
Talin variant P229S compromises integrin activation and associates with multifaceted clinical symptoms.
Hum Mol Genet. 2022 Jul 21:ddac163. doi: 10.1093/hmg/ddac163.
PMID: 35861643
Maria Cherian R, Prajapati C, Penttinen K, Häkli M, Koivisto JT, Pekkanen-Mattila M, Aalto-Setälä K.
Fluorescent hiPSC-derived MYH6-mScarlet cardiomyocytes for real-time tracking, imaging, and cardiotoxicity assays.
Cell Biol Toxicol. 2022 Jul 23. doi: 10.1007/s10565-022-09742-0.
PMID: 35870039
Lotila J, Hyvärinen T, Skottman H, Airas L, Narkilahti S, Hagman S.
Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient.
Stem Cell Res. 2022 Aug;63:102865. doi: 10.1016/j.scr.2022.102865.
PMID: 35843021
Koivisto M, Tolvanen TA, Toimela T, Miinalainen I, Kiviaho A, Kesseli J, Nykter M, Eklund L, Heinonen T.
Functional human cell-based vascularised cardiac tissue model for biomedical research and testing.
Sci Rep. 2022 Aug 5;12(1):13459. doi: 10.1038/s41598-022-17498-0.
PMID: 35931748
Karttunen J, Heiskanen M, Joki T, Hyysalo A, Navarro-Ferrandis V, Miettinen S, Narkilahti S, Pitkänen A.
Effect of cell culture media on extracellular vesicle secretion from mesenchymal stromal cells and neurons.
Eur J Cell Biol. 2022 Sep-Nov;101(4):151270. doi: 10.1016/j.ejcb.2022.151270.
PMID: 35987046
Räsänen N, Harju V, Joki T, Narkilahti S.
Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions.
Development. 2022 Oct 15;149(20):dev200012. doi: 10.1242/dev.200012.
PMID: 35929583
Tornberg K, Välimäki H, Valaskivi S, Mäki AJ, Jokinen M, Kreutzer J, Kallio P.
Compartmentalized organ-on-a-chip structure for spatiotemporal control of oxygen microenvironments.
Biomed Microdevices. 2022 Oct 21;24(4):34. doi: 10.1007/s10544-022-00634-y.
PMID: 36269438
Gaertner K, Michell C, Tapanainen R, Goffart S, Saari S, Soininmäki M, Dufour E, Pohjoismäki JLO.
Molecular phenotyping uncovers differences in basic housekeeping functions among closely related species of hares (Lepus spp., Lagomorpha: Leporidae).
Mol Ecol. 2022 Nov 1. doi: 10.1111/mec.16755.
PMID: 36320183
Kukkonen K, Autio-Kimura B, Rauhala H, Kesseli J, Nykter M, Latonen L, Visakorpi T.
Nonmalignant AR-positive prostate epithelial cells and cancer cells respond differently to androgen.
Endocr Relat Cancer. 2022 Nov 7;29(12):717-733. doi: 10.1530/ERC-22-0108.
PMID: 36219867
Gering C, Párraga J, Vuorenpää H, Botero L, Miettinen S, Kellomäki M.
Bioactivated gellan gum hydrogels affect cellular rearrangement and cell response in vascular co-culture and subcutaneous implant models.
Biomater Adv. 2022 Dec;143:213185. doi: 10.1016/j.bioadv.2022.213185.
PMID: 36371972
Häkli M, Kreutzer J, Mäki AJ, Välimäki H, Cherian RM, Kallio P, Aalto-Setälä K, Pekkanen-Mattila M.
Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation.
Stem Cells Int. 2022 Dec 19;2022:9438281. doi: 10.1155/2022/9438281.
PMID: 36579142
Mörö A, Samanta S, Honkamäki L, Rangasami VK, Puistola P, Kauppila M, Narkilahti S, Miettinen S, Oommen O, Skottman H.
Hyaluronic acid based next generation bioink for 3D bioprinting of human stem cell derived corneal stromal model with innervation.
Biofabrication. 2022 Dec 29;15(1). doi: 10.1088/1758-5090/acab34.
PMID: 36579828
2021
Tan GSW, Liu Z, Ilmarinen T, Barathi VA, Chee CK, Lingam G, Su X, Stanzel BV.
Hints for Gentle Submacular Injection in Non-Human Primates Based on Intraoperative OCT Guidance.
Transl Vis Sci Technol. 2021 Jan 7;10(1):10. doi: 10.1167/tvst.10.1.10.
PMID: 33510949
Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytönen VP.
Cancer associated talin point mutations disorganise cell adhesion and migration.
Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4.
PMID: 33431906
Viheriälä T, Sorvari J, Ihalainen TO, Mörö A, Grönroos P, Schlie-Wolter S, Chichkov B, Skottman H, Nymark S, Ilmarinen T.
Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE.
Sci Rep. 2021 Jan 13;11(1):933. doi: 10.1038/s41598-020-79638-8.
PMID: 33441679
Grönroos P, Ilmarinen T, Skottman H.
Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions.
Cells. 2021 Feb 5;10(2):331. doi: 10.3390/cells10020331.
PMID: 33562615
Honkamäki L, Joki T, Grigoryev NA, Levon K, Ylä-Outinen L, Narkilahti S.
Novel method to produce a layered 3D scaffold for human pluripotent stem cell-derived neuronal cells.
J Neurosci Methods. 2021 Feb 15;350:109043. doi: 10.1016/j.jneumeth.2020.109043.
PMID: 33345946
Häkli M, Kreutzer J, Mäki AJ, Välimäki H, Lappi H, Huhtala H, Kallio P, Aalto-Setälä K, Pekkanen-Mattila M.
Human induced pluripotent stem cell-based platform for modeling cardiac ischemia.
Sci Rep. 2021 Feb 18;11(1):4153. doi: 10.1038/s41598-021-83740-w.
PMID: 33603154
Anton-Sales I, Koivusalo L, Skottman H, Laromaine A, Roig A.
Limbal Stem Cells on Bacterial Nanocellulose Carriers for Ocular Surface Regeneration.
Small. 2021 Mar;17(10):e2003937. doi: 10.1002/smll.202003937.
PMID: 33586332
Prajapati C, Ojala M, Lappi H, Aalto-Setälä K, Pekkanen-Mattila M.
Electrophysiological evaluation of human induced pluripotent stem cell-derived cardiomyocytes obtained by different methods.
Stem Cell Res. 2021 Mar;51:102176. doi: 10.1016/j.scr.2021.102176.
PMID: 33485184
Eerola SK, Kohvakka A, Tammela TLJ, Koskinen PJ, Latonen L, Visakorpi T.
Expression and ERG regulation of PIM kinases in prostate cancer.
Cancer Med. 2021 May;10(10):3427-3436. doi: 10.1002/cam4.3893.
PMID: 33932111
George JJ, Oittinen M, Martin-Diaz L, Zapilko V, Iqbal S, Rintakangas T, Arrojo Martins FT, Niskanen H, Katajisto P, Kaikkonen MU, Viiri K.
Polycomb Repressive Complex 2 Regulates Genes Necessary for Intestinal Microfold Cell (M Cell) Development.
Cell Mol Gastroenterol Hepatol. 2021;12(3):873-889. doi: 10.1016/j.jcmgh.2021.05.014.
PMID: 34058415
Hytti M, Korhonen E, Hongisto H, Kaarniranta K, Skottman H, Kauppinen A.
Differential Expression of Inflammasome-Related Genes in Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells with or without History of Age-Related Macular Degeneration.
Int J Mol Sci. 2021 Jun 24;22(13):6800. doi: 10.3390/ijms22136800.
PMID: 34202702
Savijoki K, Myllymäki H, Luukinen H, Paulamäki L, Vanha-Aho LM, Svorjova A, Miettinen I, Fallarero A, Ihalainen TO, Yli-Kauhaluoma J, Nyman TA, Parikka M.
Surface-Shaving Proteomics of Mycobacterium marinum Identifies Biofilm Subtype-Specific Changes Affecting Virulence, Tolerance, and Persistence.
mSystems. 2021 Jun 29;6(3):e0050021. doi: 10.1128/mSystems.00500-21.
PMID: 34156290
Liu Z, Ilmarinen T, Tan GSW, Hongisto H, Wong EYM, Tsai ASH, Al-Nawaiseh S, Holder GE, Su X, Barathi VA, Skottman H, Stanzel BV.
Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model.
Stem Cell Res Ther. 2021 Jul 27;12(1):423. doi: 10.1186/s13287-021-02395-6.
PMID: 34315534
Azizi L, Turkki P, Huynh N, Massera JM, Hytönen VP.
Surface Modification of Bioactive Glass Promotes Cell Attachment and Spreading.
ACS Omega. 2021 Aug 25;6(35):22635-22642. doi: 10.1021/acsomega.1c02669.
PMID: 34514235
George JJ, Martin-Diaz L, Ojanen MJT, Gasa R, Pesu M, Viiri K.
PRC2 Regulated Atoh8 Is a Regulator of Intestinal Microfold Cell (M Cell) Differentiation.
Int J Mol Sci. 2021 Aug 28;22(17):9355. doi: 10.3390/ijms22179355.
PMID: 34502262
Hyunh NB, Palma CSD, Rahikainen R, Mishra A, Azizi L, Verne E, Ferraris S, Hytönen VP, Sanches Ribeiro A, Massera J.
Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption.
ACS Biomater Sci Eng. 2021 Sep 13;7(9):4483-4493. doi: 10.1021/acsbiomaterials.1c00735.
PMID: 34382772
Mäntylä E, Ihalainen TO.
Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells.
Sci Rep. 2021 Sep 16;11(1):18520. doi: 10.1038/s41598-021-97900-5.
PMID: 34531455
Juntunen M, Heinonen S, Huhtala H, Rissanen A, Kaprio J, Kuismanen K, Pietiläinen KH, Miettinen S, Patrikoski M.
Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs.
Stem Cell Res Ther. 2021 Sep 26;12(1):516. doi: 10.1186/s13287-021-02587-0.
PMID: 34565451
Leppiniemi J, Mutahir Z, Dulebo A, Mikkonen P, Nuopponen M, Turkki P, Hytönen VP.
Avidin-Conjugated Nanofibrillar Cellulose Hydrogel Functionalized with Biotinylated Fibronectin and Vitronectin Promotes 3D Culture of Fibroblasts.
Biomacromolecules. 2021 Oct 11;22(10):4122-4137. doi: 10.1021/acs.biomac.1c00579.
PMID: 34542997
Coentro JQ, May U, Prince S, Zwaagstra J, Ritvos O, Järvinen TAH, Zeugolis DI.
Adapting the Scar-in-a-Jar to Skin Fibrosis and Screening Traditional and Contemporary Anti-Fibrotic Therapies.
Front Bioeng Biotechnol. 2021 Oct 26;9:756399. doi: 10.3389/fbioe.2021.756399.
PMID: 34765594
Ahtiainen A, Genocchi B, Tanskanen JMA, Barros MT, Hyttinen JAK, Lenk K.
Astrocytes Exhibit a Protective Role in Neuronal Firing Patterns under Chemically Induced Seizures in Neuron-Astrocyte Co-Cultures.
Int J Mol Sci. 2021 Nov 25;22(23):12770. doi: 10.3390/ijms222312770.
PMID: 34884577
Buchacher T, Honkimaa A, Välikangas T, Lietzén N, Hirvonen MK, Laiho JE, Sioofy-Khojine AB, Eskelinen EL, Hyöty H, Elo LL, Lahesmaa R.
Persistent coxsackievirus B1 infection triggers extensive changes in the transcriptome of human pancreatic ductal cells.
iScience. 2021 Dec 18;25(1):103653. doi: 10.1016/j.isci.2021.103653.
PMID: 35024587
Vattulainen M, Ilmarinen T, Viheriälä T, Jokinen V, Skottman H.
Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5+ and ∆Np63α+ cells with limbal cell characteristics and high wound healing capacity.
Stem Cell Res Ther. 2021 Dec 20;12(1):609. doi: 10.1186/s13287-021-02673-3.
PMID: 34930437
Järvelä-Stölting M, Vesala L, Maasdorp MK, Ciantar J, Rämet M, Valanne S.
Proteasome α6 Subunit Negatively Regulates the JAK/STAT Pathway and Blood Cell Activation in Drosophila melanogaster.
Front Immunol. 2021 Dec 22;12:729631. doi: 10.3389/fimmu.2021.729631.
PMID: 35003057
Hernández-Pérez S, Runsala M, Šuštar V, Mattila PK.
Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight.
Methods Mol Biol. 2021;2304:173-191. doi: 10.1007/978-1-0716-1402-0_9.
PMID: 34028717
2020
Calejo MT, Saari J, Vuorenpää H, Vuorimaa-Laukkanen E, Kallio P, Aalto-Setälä K, Miettinen S, Skottman H, Kellomäki M, Juuti-Uusitalo K.
Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films.
Acta Biomater. 2020 Jan 1;101:327-343. doi: 10.1016/j.actbio.2019.11.002.
PMID: 31711900
Halonen HT, Ihalainen TO, Hyväri L, Miettinen S, Hyttinen JAK.
Cell adhesion and culture medium dependent changes in the high frequency mechanical vibration induced proliferation, osteogenesis, and intracellular organization of human adipose stem cells.
J Mech Behav Biomed Mater. 2020 Jan;101:103419. doi: 10.1016/j.jmbbm.2019.103419.
PMID: 31518945
Harjula SE, Saralahti AK, Ojanen MJT, Rantapero T, Uusi-Mäkelä MIE, Nykter M, Lohi O, Parikka M, Rämet M.
Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio).
Dev Comp Immunol. 2020 Feb;103:103523. doi: 10.1016/j.dci.2019.103523.
PMID: 31626817
Kreutzer J, Viehrig M, Pölönen RP, Zhao F, Ojala M, Aalto-Setälä K, Kallio P.
Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes.
Biomech Model Mechanobiol. 2020 Feb;19(1):291-303. doi: 10.1007/s10237-019-01211-8.
PMID: 31444593
Shah D, Prajapati C, Penttinen K, Cherian RM, Koivumäki JT, Alexanova A, Hyttinen J, Aalto-Setälä K.
hiPSC-Derived Cardiomyocyte Model of LQT2 Syndrome Derived from Asymptomatic and Symptomatic Mutation Carriers Reproduces Clinical Differences in Aggregates but Not in Single Cells.
Cells. 2020 May 7;9(5):1153. doi: 10.3390/cells9051153.
PMID: 32392813
Välimäki H, Hyvärinen T, Leivo J, Iftikhar H, Pekkanen-Mattila M, Rajan DK, Verho J, Kreutzer J, Ryynänen T, Pirhonen J, Aalto-Setälä K, Kallio P, Narkilahti S, Lekkala J.
Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity.
Biomed Microdevices. 2020 Jun 3;22(2):41. doi: 10.1007/s10544-020-00495-3.
PMID: 32494857
Kohvakka A, Sattari M, Shcherban A, Annala M, Urbanucci A, Kesseli J, Tammela TLJ, Kivinummi K, Latonen L, Nykter M, Visakorpi T.
AR and ERG drive the expression of prostate cancer specific long noncoding RNAs.
Oncogene. 2020 Jul;39(30):5241-5251. doi: 10.1038/s41388-020-1365-6.
PMID: 32555329
Pemmari T, Ivanova L, May U, Lingasamy P, Tobi A, Pasternack A, Prince S, Ritvos O, Makkapati S, Teesalu T, Cairo MS, Järvinen TAH, Liao Y.
Exposed CendR Domain in Homing Peptide Yields Skin-Targeted Therapeutic in Epidermolysis Bullosa.
Mol Ther. 2020 Aug 5;28(8):1833-1845. doi: 10.1016/j.ymthe.2020.05.017.
PMID: 32497513
Fedele C, Mäntylä E, Belardi B, Hamkins-Indik T, Cavalli S, Netti PA, Fletcher DA, Nymark S, Priimagi A, Ihalainen TO.
Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells.
Sci Rep. 2020 Sep 18;10(1):15329. doi: 10.1038/s41598-020-71567-w.
PMID: 32948792
Hyväri L, Vanhatupa S, Halonen HT, Kääriäinen M, Miettinen S.
Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells.
Stem Cells Int. 2020 Sep 22;2020:8853541. doi: 10.1155/2020/8853541.
PMID: 33029150
Hongisto H, Dewing JM, Christensen DR, Scott J, Cree AJ, Nättinen J, Määttä J, Jylhä A, Aapola U, Uusitalo H, Kaarniranta K, Ratnayaka JA, Skottman H, Lotery AJ.
In vitro stem cell modelling demonstrates a proof-of-concept for excess functional mutant TIMP3 as the cause of Sorsby fundus dystrophy.
J Pathol. 2020 Oct;252(2):138-150. doi: 10.1002/path.5506.
PMID: 32666594
Tamminen I, Lehto K, Hannula M, Ojansivu M, Johansson L, Kellomäki M, Miettinen S, Aula A, Ihalainen T, Hyttinen J.
A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samples.
Commun Biol. 2020 Oct 2;3(1):548. doi: 10.1038/s42003-020-01273-w.
PMID: 33009501
Juntunen M, Hagman S, Moisan A, Narkilahti S, Miettinen S.
In Vitro Oxygen-Glucose Deprivation-Induced Stroke Models with Human Neuroblastoma Cell- and Induced Pluripotent Stem Cell-Derived Neurons.
Stem Cells Int. 2020 Oct 29;2020:8841026. doi: 10.1155/2020/8841026.
PMID: 33178286
Honkimaa A, Kimura B, Sioofy-Khojine AB, Lin J, Laiho J, Oikarinen S, Hyöty H.
Genetic Adaptation of Coxsackievirus B1 during Persistent Infection in Pancreatic Cells.
Microorganisms. 2020 Nov 15;8(11):1790. doi: 10.3390/microorganisms8111790.
PMID: 33203081
Pelkonen A, Mzezewa R, Sukki L, Ryynänen T, Kreutzer J, Hyvärinen T, Vinogradov A, Aarnos L, Lekkala J, Kallio P, Narkilahti S.
A modular brain-on-a-chip for modelling epileptic seizures with functionally connected human neuronal networks.
Biosens Bioelectron. 2020 Nov 15;168:112553. doi: 10.1016/j.bios.2020.112553.
PMID: 32877779
Jäntti T, Luhtala S, Mäenpää J, Staff S.
Characterization of immunoreactivity with whole-slide imaging and digital analysis in high-grade serous ovarian cancer.
Tumour Biol. 2020 Nov;42(11):1010428320971404. doi: 10.1177/1010428320971404.
PMID: 33169632
Rasheed A, Azizi L, Turkki P, Janka M, Hytönen VP, Tuukkanen S.
Extrusion-Based Bioprinting of Multilayered Nanocellulose Constructs for Cell Cultivation Using In Situ Freezing and Preprint CaCl2 Cross-Linking.
ACS Omega. 2020 Dec 30;6(1):569-578. doi: 10.1021/acsomega.0c05036.
PMID: 33458509
2019
Karvinen J, Ihalainen TO, Calejo MT, Jönkkäri I, Kellomäki M.
Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studies.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:1056-1066. doi: 10.1016/j.msec.2018.10.048.
PMID: 30423686
Valanne S, Salminen TS, Järvelä-Stölting M, Vesala L, Rämet M.
Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster.
PLoS Pathog. 2019 Jan 11;15(1):e1007504. doi: 10.1371/journal.ppat.1007504.
PMID: 30633769
Korkka I, Viheriälä T, Juuti-Uusitalo K, Uusitalo-Järvinen H, Skottman H, Hyttinen J, Nymark S.
Functional Voltage-Gated Calcium Channels Are Present in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium.
Stem Cells Transl Med. 2019 Feb;8(2):179-193. doi: 10.1002/sctm.18-0026.
PMID: 30394009
Viiri LE, Rantapero T, Kiamehr M, Alexanova A, Oittinen M, Viiri K, Niskanen H, Nykter M, Kaikkonen MU, Aalto-Setälä K.
Extensive reprogramming of the nascent transcriptome during iPSC to hepatocyte differentiation.
Sci Rep. 2019 Mar 5;9(1):3562. doi: 10.1038/s41598-019-39215-0.
PMID: 30837492
González de Cózar JM, Gerards M, Teeri E, George J, Dufour E, Jacobs HT, Jõers P.
RNase H1 promotes replication fork progression through oppositely transcribed regions of Drosophila mitochondrial DNA.
J Biol Chem. 2019 Mar 22;294(12):4331-4344. doi: 10.1074/jbc.RA118.007015.
PMID: 30635398
Aspatwar A, Tolvanen MEE, Schneider HP, Becker HM, Narkilahti S, Parkkila S, Deitmer JW.
Catalytically-inactive carbonic anhydrase-related proteins enhance transport of lactate by MCT1.
FEBS Open Bio. 2019 Apr 29. doi: 10.1002/2211-5463.12647.
PMID: 31033227
Rahikainen R, Öhman T, Turkki P, Varjosalo M, Hytönen VP.
Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
J Cell Sci. 2019 Apr 3;132(7). pii: jcs226514. doi: 10.1242/jcs.226514.
PMID: 30837291
Hammarén HM, Virtanen AT, Abraham BG, Peussa H, Hubbard SR, Silvennoinen O.
Janus kinase 2 activation mechanisms revealed by analysis of suppressing mutations.
J Allergy Clin Immunol. 2019 Apr;143(4):1549-1559.e6. doi: 10.1016/j.jaci.2018.07.022.
PMID: 30092288
Alarautalahti V, Ragauskas S, Hakkarainen JJ, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S.
Viability of Mouse Retinal Explant Cultures Assessed by Preservation of Functionality and Morphology.
Invest Ophthalmol Vis Sci. 2019 May 1;60(6):1914-1927. doi: 10.1167/iovs.18-25156.
PMID: 31042799
Pekkanen-Mattila M, Häkli M, Pölönen RP, Mansikkala T, Junnila A, Talvitie E, Koivisto JT, Kellomäki M, Aalto-Setälä K.
Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Materials (Basel). 2019 Jun 3;12(11). pii: E1805. doi: 10.3390/ma12111805.
PMID: 31163704
Koivisto JT, Gering C, Karvinen J, Maria Cherian R, Belay B, Hyttinen J, Aalto-Setälä K, Kellomäki M, Parraga J.
Mechanically Biomimetic Gelatin-Gellan Gum Hydrogels for 3D Culture of Beating Human Cardiomyocytes.
ACS Appl Mater Interfaces. 2019 Jun 12;11(23):20589-20602. doi: 10.1021/acsami.8b22343.
PMID: 31120238
Vuornos K, Ojansivu M, Koivisto JT, Häkkänen H, Belay B, Montonen T, Huhtala H, Kääriäinen M, Hupa L, Kellomäki M, Hyttinen J, Ihalainen JA, Miettinen S.
Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:905-918. doi: 10.1016/j.msec.2019.02.035.
PMID: 30889765
Aspatwar A, Tolvanen MEE, Schneider HP, Becker HM, Narkilahti S, Parkkila S, Deitmer JW.
Catalytically inactive carbonic anhydrase-related proteins enhance transport of lactate by MCT1.
FEBS Open Bio. 2019 Jul;9(7):1204-1211. doi: 10.1002/2211-5463.12647.
PMID: 31033227
Ylä-Outinen L, Harju V, Joki T, Koivisto JT, Karvinen J, Kellomäki M, Narkilahti S.
Screening of Hydrogels for Human Pluripotent Stem Cell-Derived Neural Cells: Hyaluronan-Polyvinyl Alcohol-Collagen-Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold.
Macromol Biosci. 2019 Jul;19(7):e1900096. doi: 10.1002/mabi.201900096.
PMID: 31173471
Kiamehr M, Klettner A, Richert E, Koskela A, Koistinen A, Skottman H, Kaarniranta K, Aalto-Setälä K, Juuti-Uusitalo K.
Compromised Barrier Function in Human Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells from Type 2 Diabetic Patients.
Int J Mol Sci. 2019 Aug 1;20(15). pii: E3773. doi: 10.3390/ijms20153773.
PMID: 31375001
Vattulainen M, Ilmarinen T, Koivusalo L, Viiri K, Hongisto H, Skottman H.
Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential.
Stem Cell Res Ther. 2019 Aug 5;10(1):236. doi: 10.1186/s13287-019-1354-2.
PMID:31383008
Johansson JK, Karema-Jokinen VI, Hakanen S, Jylhä A, Uusitalo H, Vihinen-Ranta M, Skottman H, Ihalainen TO, Nymark S.
Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium.
BMC Biol. 2019 Aug 15;17(1):63. doi: 10.1186/s12915-019-0681-1.
PMID: 31412898
Gering C, Koivisto JT, Parraga J, Leppiniemi J, Vuornos K, Hytönen VP, Miettinen S, Kellomäki M.
Design of modular gellan gum hydrogel functionalized with avidin and biotinylated adhesive ligands for cell culture applications.
PLoS One. 2019 Aug 30;14(8):e0221931. doi: 10.1371/journal.pone.0221931.
PMID: 31469884
Lietzén N, Hirvonen K, Honkimaa A, Buchacher T, Laiho JE, Oikarinen S, Mazur MA, Flodström-Tullberg M, Dufour E, Sioofy-Khojine AB, Hyöty H, Lahesmaa R.
Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells.
iScience. 2019 Sep 27;19:340-357. doi: 10.1016/j.isci.2019.07.040.
PMID: 31404834
Bahhir D, Yalgin C, Ots L, Järvinen S, George J, Naudí A, Krama T, Krams I, Tamm M, Andjelković A, Dufour E, González de Cózar JM, Gerards M, Parhiala M, Pamplona R, Jacobs HT, Jõers P.
Manipulating mtDNA in vivo reprograms metabolism via novel response mechanisms.
PLoS Genet. 2019 Oct 4;15(10):e1008410. doi: 10.1371/journal.pgen.1008410.
PMID: 31584940
Harjula SE, Saralahti AK, Ojanen MJT, Rantapero T, Uusi-Mäkelä MIE, Nykter M, Lohi O, Parikka M, Rämet M.
Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio).
Dev Comp Immunol. 2019 Oct 15;103:103523. doi: 10.1016/j.dci.2019.103523.
PMID: 31626817
Calejo MT, Saari J, Vuorenpää H, Vuorimaa-Laukkanen E, Kallio P, Aalto-Setälä K, Miettinen S, Skottman H, Kellomäki M, Juuti-Uusitalo K.
Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films.
Acta Biomater. 2019 Nov 8. pii: S1742-7061(19)30742-1. doi: 10.1016/j.actbio.2019.11.002.
PMID: 31711900
Hyvärinen T, Hyysalo A, Kapucu F.E, Aarnos L, Vinogradov A, Eglen S.J, Ylä-Outinen L, Narkilahti S.
Co-stimulation with IL-1β and TNF-α induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system.
Sci Rep. 2019 Nov 15;9(1):16944. doi: 10.1038/s41598-019-53414-9.
PMID: 31729450
Hyvärinen T, Hyysalo A, Kapucu FE, Aarnos L, Vinogradov A, Eglen SJ, Ylä-Outinen L, Narkilahti S.
Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures.
Sci Rep. 2019 Nov 20;9(1):17125. doi: 10.1038/s41598-019-53647-8.
PMID: 31748598
Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytönen VP, Howarth M.
Approaching infinite affinity through engineering of peptide-protein interaction.
Proc Natl Acad Sci U S A. 2019 Dec 10;116(52):26523-33. doi: 10.1073/pnas.1909653116.
PMID: 31822621
Koivusalo L, Kauppila M, Samanta S, Parihar VS, Ilmarinen T, Miettinen S, Oommen OP, Skottman H.
Tissue adhesive hyaluronic acid hydrogels for sutureless stem cell delivery and regeneration of corneal epithelium and stroma.
Biomaterials. 2019 Dec;225:119516. doi: 10.1016/j.biomaterials.2019.119516.
PMID: 31574405
2018
Saarinen NVV, Laiho JE, Richardson SJ, Zeissler M, Stone VM, Marjomäki V, Kantoluoto T, Horwitz MS, Sioofy-Khojine A, Honkimaa A, Hankaniemi MM, Flodström-Tullberg M, Hyöty H, Hytönen VP, Laitinen OH.
A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses.
Sci Rep. 2018 Jan 8;8(1):33. doi: 10.1038/s41598-017-18495-4.
PMID: 29311608
Gerards M, Cannino G, de Cózar JMG, Jacobs HT.
Intracellular vesicle trafficking plays an essential role in mitochondrial quality control.
Mol Biol Cell. 2018 Jan 17. pii: mbc.E17-10-0619. doi: 10.1091/mbc.E17-10-0619.
PMID: 29343549
Ojansivu M, Wang X, Hyväri L, Kellomäki M, Hupa L, Vanhatupa S, Miettinen S.
Bioactive glass induced osteogenic differentiation of human adipose stem cells is dependent on cell attachment mechanism and mitogen-activated protein kinases.
Eur Cell Mater. 2018 Jan; 35:54-72. doi: 10.22203/eCM.v035a05.
PMID: 29383695
Vähätupa M, Cordova ZM, Barker H, Aittomäki S, Uusitalo H, Järvinen TAH, Pesu M, Uusitalo-Järvinen H.
Furin deficiency in myeloid cells leads to attenuated revascularization in a mouse-model of oxygen-induced retinopathy.
Exp Eye Res. 2018 Jan;166:160-167. doi: 10.1016/j.exer.2017.10.013.
PMID: 29031855
Aspatwar A, Haapanen S, Parkkila S.
An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.
Metabolites. 2018 Mar 13;8(1). pii: E22. doi: 10.3390/metabo8010022.
PMID: 29534024
Vuorinen EM, Rajala NK, Ihalainen TO, Kallioniemi A.
Depletion of nuclear import protein karyopherin alpha 7 (KPNA7) induces mitotic defects and deformation of nuclei in cancer cells.
BMC Cancer. 2018 Mar 27;18(1):325. doi: 10.1186/s12885-018-4261-5.
PMID: 29580221
Paavilainen T, Pelkonen A, Mäkinen ME, Peltola M, Huhtala H, Fayuk D, Narkilahti S.
Effect of prolonged differentiation on functional maturation of human pluripotent stem cell-derived neuronal cultures.
Stem Cell Res. 2018 Mar;27:151-161. doi: 10.1016/j.scr.2018.01.018.
PMID: 29414606
Koivusalo L, Karvinen J, Sorsa E, Jönkkäri I, Väliaho J, Kallio P, Ilmarinen T, Miettinen S, Skottman H, Kellomäki M.
Hydrazone crosslinked hyaluronan-based hydrogels for therapeutic delivery of adipose stem cells to treat corneal defects.
Mater Sci Eng C Mater Biol Appl. 2018 Apr 1;85:68-78. doi: 10.1016/j.msec.2017.12.013.
PMID: 29407158
Ojansivu M, Johansson L, Vanhatupa S, Tamminen I, Hannula M, Hyttinen J, Kellomäki M, Miettinen S.
Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis.
Stem Cells Int. 2018 May 7;2018:5928935. doi: 10.1155/2018/5928935.
PMID: 29853915
Vähätupa M, Nättinen J, Jylhä A, Aapola U, Kataja M, Kööbi P, Järvinen TAH, Uusitalo H, Uusitalo-Järvinen H.
SWATH-MS Proteomic Analysis of Oxygen-Induced Retinopathy Reveals Novel Potential Therapeutic Targets.
Invest Ophthalmol Vis Sci. 2018 Jul 2;59(8):3294-3306. doi: 10.1167/iovs.18-23831.
PMID: 30025079
Prajapati C, Pölönen RP, Aalto-Setälä K.
Simultaneous recordings of action potentials and calcium transients from human induced pluripotent stem cell derived cardiomyocytes.
Biol Open. 2018 Jul 16;7(7). pii: bio035030. doi: 10.1242/bio.035030.
PMID: 29970475
Abu Khamidakh AE, Rodriguez-Martinez A, Kaarniranta K, Kallioniemi A, Skottman H, Hyttinen J, Juuti-Uusitalo K.
Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage.
Biomed Eng Online. 2018 Jul 31;17(1):102. doi: 10.1186/s12938-018-0535-z.
PMID: 30064430
Sorkio A, Koch L, Koivusalo L, Deiwick A, Miettinen S, Chichkov B, Skottman H.
Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks.
Biomaterials. 2018 Jul;171:57-71. doi: 10.1016/j.biomaterials.2018.04.034.
PMID: 29684677
Hammarén HM, Virtanen AT, Abraham BG, Peussa H, Hubbard SR, Silvennoinen O.
Janus kinase 2 activation mechanisms revealed by analysis of suppressing mutations.
J Allergy Clin Immunol. 2018 Aug 6. pii: S0091-6749(18)31129-1. doi: 10.1016/j.jaci.2018.07.022.
PMID: 30092288
Sartoneva R, Kuismanen K, Juntunen M, Karjalainen S, Hannula M, Kyllönen L, Hyttinen J, Huhtala H, Paakinaho K, Miettinen S.
Porous poly-l-lactide-co-ɛ-caprolactone scaffold: a novel biomaterial for vaginal tissue engineering.
R Soc Open Sci. 2018 Aug 15;5(8):180811. doi: 10.1098/rsos.180811.
PMID: 30225072
Ojansivu M, Mishra A, Vanhatupa S, Juntunen M, Larionova A, Massera J, Miettinen S.
The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells.
PLoS One. 2018 Aug 28;13(8):e0202740. doi: 10.1371/journal.pone.0202740.
PMID: 30153295
Hagman S, Mäkinen A, Ylä-Outinen L, Huhtala H, Elovaara I, Narkilahti S.
Effects of inflammatory cytokines IFN-γ, TNF-α and IL-6 on the viability and functionality of human pluripotent stem cell-derived neural cells.
J Neuroimmunol. 2018 Aug 29. pii: S0165-5728(17)30480-0. doi: 10.1016/j.jneuroim.2018.07.010.
PMID: 30195439
Hyväri L, Ojansivu M, Juntunen M, Kartasalo K, Miettinen S, Vanhatupa S.
Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages.
Stem Cells Int. 2018 Sep 12;2018:2190657. doi: 10.1155/2018/2190657.
PMID: 30275837
Hongisto H, Vattulainen M, Ilmarinen T, Mikhailova A, Skottman H.
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells.
J Vis Exp. 2018 Oct 24;(140). doi: 10.3791/58279.
PMID: 30417867
Andjelković A, Mordas A, Bruinsma L, Ketola A, Cannino G, Giordano L, Dhandapani PK, Szibor M, Dufour E, Jacobs HT.
Expression of the Alternative Oxidase Influences Jun N-Terminal Kinase Signaling and Cell Migration.
Mol Cell Biol. 2018 Nov 28;38(24). pii: e00110-18. doi: 10.1128/MCB.00110-18.
PMID: 30224521
Ryynänen T, Toivanen M, Salminen T, Ylä-Outinen L, Narkilahti S, Lekkala J.
Ion Beam Assisted E-Beam Deposited TiN Microelectrodes-Applied to Neuronal Cell Culture Medium Evaluation.
Front Neurosci. 2018 Dec 4;12:882. doi: 10.3389/fnins.2018.00882.
PMID: 30568570
Ilmarinen T, Thieltges F, Hongisto H, Juuti-Uusitalo K, Koistinen A, Kaarniranta K, Brinken R, Braun N, Holz FG, Skottman H, Stanzel BV.
Survival and functionality of xeno-free human embryonic stem cell-derived retinal pigment epithelial cells on polyester substrate after transplantation in rabbits.
Acta Ophthalmol. 2018 Dec 28. doi: 10.1111/aos.14004.
PMID: 30593729
2017
Kumar M, Rahikainen R, Unruh D, Hytönen VP, Delbrück C, Sindelar R, Renz F.
Mixture of PLA-PEG and biotinylated albumin enables immobilization of avidins on electrospun fibers.
J Biomed Mater Res A. 2017 Feb;105(2):356-362. doi: 10.1002/jbm.a.35920.
PMID: 27684712
Kreutzer J, Ylä-Outinen L, Mäki AJ, Ristola M, Narkilahti S, Kallio P.
Cell culture chamber with gas supply for prolonged recording of human neuronal cells on microelectrode array.
J Neurosci Methods. 2017 Mar 15;280:27-35. doi: 10.1016/j.jneumeth.2017.01.019.
PMID: 28161299
Koivisto JT, Joki T, Parraga JE, Pääkkönen R, Ylä-Outinen L, Salonen L, Jönkkäri I, Peltola M, Ihalainen TO, Narkilahti S, Kellomäki M.
Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering.
Biomed Mater. 2017 Mar 24;12(2):025014. doi: 10.1088/1748-605X/aa62b0.
PMID: 28233757
Juuti-Uusitalo K, Koskela A, Kivinen N, Viiri J, Hyttinen JMT, Reinisalo M, Koistinen A, Uusitalo H, Sinha D, Skottman H, Kaarniranta K.
Autophagy Regulates Proteasome Inhibitor-Induced Pigmentation in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.
Int J Mol Sci. 2017 May 19;18(5). pii: E1089. doi: 10.3390/ijms18051089.
PMID: 28534814
Oksala NKJ, Seppälä I, Rahikainen R, Mäkelä KM, Raitoharju E, Illig T, Klopp N, Kholova I, Laaksonen R, Karhunen PJ, Hytönen VP, Lehtimäki T.
Synergistic Expression of Histone Deacetylase 9 and Matrix Metalloproteinase 12 in M4 Macrophages in Advanced Carotid Plaques.
Eur J Vasc Endovasc Surg. 2017 May;53(5):632-640. doi: 10.1016/j.ejvs.2017.02.014.
PMID: 28343758
Calejo MT, Ilmarinen T, Vuorimaa-Laukkanen E, Talvitie E, Hakola HM, Skottman H, Kellomäki M.
Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering.
Acta Biomater. 2017 May;54:138-149. doi: 10.1016/j.actbio.2017.02.035.
PMID: 28223209
Rahikainen R, von Essen M, Schaefer M, Qi L, Azizi L, Kelly C, Ihalainen TO, Wehrle-Haller B, Bastmeyer M, Huang C, Hytönen VP.
Mechanical stability of talin rod controls cell migration and substrate sensing.
Sci Rep. 2017 Jun 15;7(1):3571. doi: 10.1038/s41598-017-03335-2.
PMID: 2862017
Saari S, Andjelković A, Garcia GS, Jacobs HT, Oliveira MT.
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster.
BMC Dev Biol. 2017 Jul 3;17(1):9. doi: 10.1186/s12861-017-0151-3.
PMID: 28673232
Leppiniemi J, Lahtinen P, Paajanen A, Mahlberg R, Metsä-Kortelainen S, Pinomaa T, Pajari H, Vikholm-Lundin I, Pursula P, Hytönen VP.
3D-Printable Bioactivated Nanocellulose-Alginate Hydrogels.
ACS Appl Mater Interfaces. 2017 Jul 5;9(26):21959-21970. doi: 10.1021/acsami.7b02756.
PMID: 28598154
Leivo J, Virjula S, Vanhatupa S, Kartasalo K, Kreutzer J, Miettinen S, Kallio P.
A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture.
J R Soc Interface. 2017 Jul;14(132). pii: 20170318. doi: 10.1098/rsif.2017.0318.
PMID: 28747398
Hyysalo A, Ristola M, Joki T, Honkanen M, Vippola M, Narkilahti S.
Aligned Poly(ε-caprolactone) Nanofibers Guide the Orientation and Migration of Human Pluripotent Stem Cell-Derived Neurons, Astrocytes, and Oligodendrocyte Precursor Cells In Vitro.
Macromol Biosci. 2017 Jul;17(7). doi: 10.1002/mabi.201600517.
PMID: 28296144
Skottman H, Muranen J, Lähdekorpi H, Pajula E, Mäkelä K, Koivusalo L, Koistinen A, Uusitalo H, Kaarniranta K, Juuti-Uusitalo K.
Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells.
Exp Cell Res. 2017 Aug 8. pii: S0014-4827(17)30415-9. doi: 10.1016/j.yexcr.2017.08.004.
PMID: 28800962
Turunen S, Joki T, Hiltunen ML, Ihalainen TO, Narkilahti S, Kellomäki M.
Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells.
ACS Appl Mater Interfaces. 2017 Aug 9;9(31):25717-25730. doi: 10.1021/acsami.7b05536.
PMID: 28697300
Virjula S, Zhao F, Leivo J, Vanhatupa S, Kreutzer J, Vaughan TJ, Honkala AM, Viehrig M, Mullen CA, Kallio P, McNamara LM, Miettinen S.
The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells.
J Mech Behav Biomed Mater. 2017 Aug;72:38-48. doi: 10.1016/j.jmbbm.2017.04.016.
PMID: 28448920
Kiamehr M, Viiri LE, Vihervaara T, Koistinen KM, Hilvo M, Ekroos K, Käkelä R, Aalto-Setälä K.
Lipidomic profiling of patient-specific iPSC-derived hepatocyte-like cells.
Dis Model Mech. 2017 Sep 1;10(9):1141-1153. doi: 10.1242/dmm.030841.
PMID: 28733363
Hyysalo A, Ristola M, Mäkinen ME, Häyrynen S, Nykter M, Narkilahti S.
Laminin α5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro.
Stem Cell Res. 2017 Sep 12;24:118-127. doi: 10.1016/j.scr.2017.09.002.
PMID: 28926760
Vuorenpää H, Penttinen K, Heinonen T, Pekkanen-Mattila M, Sarkanen JR, Ylikomi T, Aalto-Setälä K.
Maturation of human pluripotent stem cell derived cardiomyocytes is improved in cardiovascular construct.
Cytotechnology. 2017 Oct;69(5):785-800. doi: 10.1007/s10616-017-0088-1.
PMID: 28397099
Sorkio A, Haimi S, Verdoold V, Juuti-Uusitalo K, Grijpma D, Skottman H.
Poly(trimethylene carbonate) as an elastic biodegradable film for human embryonic stem cell-derived retinal pigment epithelial cells.
J Tissue Eng Regen Med. 2017 Nov;11(11):3134-3144. doi: 10.1002/term.2221.
PMID:28052579
Hongisto H, Ilmarinen T, Vattulainen M, Mikhailova A, Skottman H.
Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method.
Stem Cell Res Ther. 2017 Dec 29;8(1):291. doi: 10.1186/s13287-017-0738-4.
PMID:29284513
2016
Kemppainen E, George J, Garipler G, Tuomela T, Kiviranta E, Soga T, Dunn CD, Jacobs HT.
Mitochondrial Dysfunction Plus High-Sugar Diet Provokes a Metabolic Crisis That Inhibits Growth.
PLoS One. 2016 Mar 8;11(3):e0151421. doi: 10.1371/journal.pone.0151421.
PMID: 26954062
Vuornos K, Björninen M, Talvitie E, Paakinaho K, Kellomäki M, Huhtala H, Miettinen S, Seppänen-Kaijansinkko R, Haimi S.
Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds Is a Potential Approach for Tendon Tissue Engineering.
Tissue Eng Part A. 2016 Mar;22(5-6):513-23. doi: 10.1089/ten.tea.2015.0276.
PMID: 26919401
Calejo MT, Ilmarinen T, Jongprasitkul H, Skottman H, Kellomäki M.
Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium.
J Biomed Mater Res A. 2016 Jul;104(7):1646-56. doi: 10.1002/jbm.a.35690.
PMID: 26914698
Anderl I, Vesala L, Ihalainen TO, Vanha-Aho LM, Andó I, Rämet M, Hultmark D.
Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection.
PLoS Pathog. 2016 Jul 14;12(7):e1005746. doi: 10.1371/journal.ppat.1005746.
PMID: 27414410
Hynninen V, Vuori L, Hannula M, Tapio K, Lahtonen K, Isoniemi T, Lehtonen E, Hirsimäki M, Toppari JJ, Valden M, Hytönen VP.
Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology.
Sci Rep. 2016 Jul 6;6:29324. doi: 10.1038/srep29324.
PMID: 27381834
Lenk K, Priwitzer B, Ylä-Outinen L, Tietz LH, Narkilahti S, Hyttinen JA.
Simulation of developing human neuronal cell networks.
Biomed Eng Online. 2016 Aug 30;15(1):105. doi: 10.1186/s12938-016-0226-6.
PMID: 27576323
Andjelković A, Kemppainen KK, Jacobs HT.
Ligand-Bound GeneSwitch Causes Developmental Aberrations in Drosophila that Are Alleviated by the Alternative Oxidase.
G3 (Bethesda). 2016 Sep 8;6(9):2839-46. doi: 10.1534/g3.116.030882.
PMID: 27412986
von Essen M, Rahikainen R, Oksala N, Raitoharju E, Seppälä I, Mennander A, Sioris T, Kholová I, Klopp N, Illig T, Karhunen PJ, Kähönen M, Lehtimäki T, Hytönen VP.
Talin and vinculin are downregulated in atherosclerotic plaque; Tampere Vascular Study.
Atherosclerosis. 2016 Dec;255:43-53. doi: 10.1016/j.atherosclerosis.2016.10.031.
PMID: 27816808
2015
Faia-Torres AB, Goren T, Ihalainen TO, Guimond-Lischer S, Charnley M, Rottmar M, Maniura-Weber K, Spencer ND, Reis RL, Textor M, Neves NM.
Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: a gradient study.
ACS Appl Mater Interfaces. 2015 Feb 4;7(4):2367-75. doi: 10.1021/am506951c.
PMID: 25513839
Vanha-Aho LM, Anderl I, Vesala L, Hultmark D, Valanne S, Rämet M.
Edin Expression in the Fat Body Is Required in the Defense Against Parasitic Wasps in Drosophila melanogaster.
PLoS Pathog. 2015 May 12;11(5):e1004895. doi: 10.1371/journal.ppat.1004895.
PMID: 25965263
Ribeiro C, Pärssinen J, Sencadas V, Correia V, Miettinen S, Hytönen VP, Lanceros-Méndez S.
Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells.
J Biomed Mater Res A. 2015 Jun;103(6):2172-5. doi: 10.1002/jbm.a.35368.
PMID: 25370596
Syrjänen L, Valanne S, Kuuslahti M, Tuomela T, Sriram A, Sanz A, Jacobs HT, Rämet M, Parkkila S.
β carbonic anhydrase is required for female fertility in Drosophila melanogaster.
Front Zool. 2015 Aug 22;12:19. doi: 10.1186/s12983-015-0111-3.
PMID: 26300950
Oksala N, Pärssinen J, Seppälä I, Klopp N, Illig T, Laaksonen R, Levula M, Raitoharju E, Kholova I, Sioris T, Kähönen M, Lehtimäki T, Hytönen VP.
Kindlin 3 (FERMT3) is associated with unstable atherosclerotic plaques, anti-inflammatory type II macrophages and upregulation of beta-2 integrins in all major arterial beds.
Atherosclerosis. 2015 Sep;242(1):145-54. doi: 10.1016/j.atherosclerosis.2015.06.058.
PMID: 26188538
Koho T, Ihalainen TO, Stark M, Uusi-Kerttula H, Wieneke R, Rahikainen R, Blazevic V, Marjomäki V, Tampé R, Kulomaa MS, Hytönen VP.
His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.
Eur J Pharm Biopharm. 2015 Oct;96:22-31. doi: 10.1016/j.ejpb.2015.07.002.
PMID: 26170162
Ihalainen TO, Aires L, Herzog FA, Schwartlander R, Moeller J, Vogel V.
Differential basal-to-apical accessibility of lamin A/C epitopes in the nuclear lamina regulated by changes in cytoskeletal tension.
Nat Mater. 2015 Dec;14(12):1252-61. doi: 10.1038/nmat4389.
PMID: 26301768
2014
Rajala N, Gerhold JM, Martinsson P, Klymov A, Spelbrink JN.
Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication.
Nucleic Acids Res. 2014 Jan;42(2):952-67. doi: 10.1093/nar/gkt988.
PMID: 24163258
Kemppainen KK, Rinne J, Sriram A, Lakanmaa M, Zeb A, Tuomela T, Popplestone A, Singh S, Sanz A, Rustin P, Jacobs HT.
Expression of alternative oxidase in Drosophila ameliorates diverse phenotypes due to cytochrome oxidase deficiency.
Hum Mol Genet. 2014 Apr 15;23(8):2078-93. doi: 10.1093/hmg/ddt601.
PMID: 24293544
Vartiainen S, Chen S, George J, Tuomela T, Luoto KR, O'Dell KM, Jacobs HT.
Phenotypic rescue of a Drosophila model of mitochondrial ANT1 disease.
Dis Model Mech. 2014 Jun;7(6):635-48. doi: 10.1242/dmm.016527.
PMID: 24812436
Fukuoh A, Cannino G, Gerards M, Buckley S, Kazancioglu S, Scialo F, Lihavainen E, Ribeiro A, Dufour E, Jacobs HT.
Screen for mitochondrial DNA copy number maintenance genes reveals essential role for ATP synthase.
Mol Syst Biol. 2014 Jun 21;10:734. doi: 10.15252/msb.20145117.
PMID: 24952591
Contacts
Imaging Systems:
Support’s shared email address:
cofamicroscopy(at)lists.tuni.fi
Laboratory Assistant:
Eija Hannuksela
Room: Arvo D443
Imaging Specialists:
Sanni Erämies
Room: Arvo D223
Outi Paloheimo
Room: Arvo D224
Management:
Teemu Ihalainen, Ph.D., Adj. Prof.
Tel: +358 503187202
Room: Arvo D337