Electrophysiological Measurements

The retinal pigment epithelium displays electrical excitability and lateral signal spreading.
Ignatova I, Frolov R, Nymark S. BMC Biol. 2023 Apr 17;21(1):84. doi: 10.1186/s12915-023-01559-5. PMID: 37069561

Heterogeneity of Potassium Channels in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium.
Korkka I, Skottman H, Nymark S. Stem Cells Transl Med. 2022 Jul 20;11(7):753-766. doi: 10.1093/stcltm/szac029. PMID: 35639962

Sex differences in heart: from basics to clinics.
Prajapati C, Koivumäki J, Pekkanen-Mattila M, Aalto-Setälä K. Eur J Med Res. 2022 Nov 9;27(1):241. doi: 10.1186/s40001-022-00880-z. PMID: 36352432

Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation.
Häkli M, Kreutzer J, Mäki AJ, Välimäki H, Cherian RM, Kallio P, Aalto-Setälä K, Pekkanen-Mattila M. Stem Cells Int. 2022 Dec 19;2022:9438281. doi: 10.1155/2022/9438281. eCollection 2022. PMID: 36579142

Data analytics for cardiac diseases.
Juhola M, Joutsijoki H, Penttinen K, Shah D, Pölönen RP, Aalto-Setälä K. Comput Biol Med. 2022 Mar;142:105218. doi: 10.1016/j.compbiomed.2022.105218. Epub 2022 Jan 5. PMID: 34999413

Fluorescent hiPSC-derived MYH6-mScarlet cardiomyocytes for real-time tracking, imaging, and cardiotoxicity assays.
Maria Cherian R, Prajapati C, Penttinen K, Häkli M, Koivisto JT, Pekkanen-Mattila M, Aalto-Setälä K. Cell Biol Toxicol. 2023 Feb;39(1):145-163. doi: 10.1007/s10565-022-09742-0. Epub 2022 Jul 23. PMID: 35870039

Martta Häkli2*, Satu Jäntti1*, Tiina Joki1, Lassi Sukki4, Kaisa Tornberg4, Katriina Aalto- Setälä2,3, Pasi Kallio4, Mari Pekkanen-Mattila2, Susanna Narkilahti1 Human neurons form axon mediated functional connections with human cardiomyocytes in compartmentalized microfluidic chip. Int. J. Mol. Sci. 2022, 23(6), 3148; https://doi.org/10.3390/ijms23063148, OA

Fikret Emre Kapucu1, Andrey Vinogradov1, Tanja Hyvärinen2, Laura Aarnos3, Laura Ylä-Outinen1, Susanna Narkilahti Comparative microelectrode array data of functional development of human PSC-derived and rat neuronal networks. Accepted to Scientific Data 3/2022. OA  

Mzezewa R, Lotila J, Kiiski H, Peltola J, Hagman S and Narkilahti S. A kainic acid-induced seizure model in human pluripotent stem cell-derived cortical neurons for studying the role of IL-6 in the functional activity. Stem Cell Research, col 60, 2022. https://doi.org/10.1016/j.scr.2022.102665, OA

Julia Fadjukov, Sophia Wienbar, Satu Hakanen, Vesa Aho, Maija Vihinen-Ranta, Teemu O. Ihalainen, Gregory W. Schwartz, Soile Nymark; Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium. J Gen Physiol 4 April 2022; 154 (4): e202112916. doi: https://doi.org/10.1085/jgp.202112916

Gaballah, M.; Penttinen, K.; Kreutzer, J.; Mäki, A.-J.; Kallio, P.; Aalto-Setälä, K. Cardiac Ischemia On-a-Chip: Antiarrhythmic Effect of Levosimendan on Ischemic Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Cells 2022, 11, 1045. https://doi.org/10.3390/cells11061045

1.    Häkli, M., Kreutzer, J., Mäki, A.-J., Välimäki, H., Lappi, H., Huhtala, H., Kallio, P., Aalto-Setälä, K. & Pekkanen-Mattila, M. (2021). Induced pluripotent stem cell-based platform for modeling cardiac ischemia, Scientific Reports, Vol. 11, No. 4153
2.    Prajapati C, Ojala M, Lappi H, Aalto-Setälä K, Pekkanen-Mattila M. Electrophysiological evaluation of human induced pluripotent stem cell-derived cardiomyocytes obtained by different methods. Stem Cell Res. 2021 Mar;51:102176. doi: 10.1016/j.scr.2021.102176. Epub 2021 Jan 15. PMID: 33485184.
 

1.    Pelkonen A, Mzezewa R, Sukki L, Ryynänen T, Kreutzer J, Hyvärinen T, Vinogradov A, Aarnos L, Lekkala J, Kallio P, Narkilahti S (2020) A modular brain-on-a-chip for modelling epileptic seizures with functionally connected human neuronal networks. Biosensors and Bioelectronics Volume 168, 15 November 2020. DOI: 10.1016/j.bios.2020.112553. 
2.    Ryynänen T, Mzezewa R, Meriläinen E, Hyvärinen T, Lekkala J, Narkilahti, S (2020) Kallio, P. Transparent Microelectrode Arrays Fabricated by Ion Beam Assisted Deposition for Neuronal Cell in Vitro Recordings. Micromachines 2020, 11, 497. DOI: 0.3390/mi11050497.
3.    Shah D, Prajapati C, Penttinen K, Cherian RM, Koivumäki JT, Alexanova A, Hyttinen J, Aalto-Setälä K. hiPSC-Derived Cardiomyocyte Model of LQT2 Syndrome Derived from Asymptomatic and Symptomatic Mutation Carriers Reproduces Clinical Differences in Aggregates but Not in Single Cells. Cells. 2020 May 7;9(5):1153. doi: 10.3390/cells9051153. PMID: 32392813; PMCID: PMC7290503.
4.    Pölönen, R.P., Swan, H. & Aalto-Setälä, K. Mutation-specific differences in arrhythmias and drug responses in CPVT patients: simultaneous patch clamp and video imaging of iPSC derived cardiomyocytes. Mol Biol Rep 47, 1067-1077 (2020). https://doi.org/10.1007/s11033-019-05201-y
 

1.    Hyvärinen T, Hyysalo A, Kapucu FE, Aarnos L, Vinogradov A, Eglen S.J, Ylä-Outinen L, Narkilahti S (2019) Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures. Sci Rep. 2019 Nov 20;9(1):17125. DOI: 10.1038/s41598-019-53647-8.
2.    Hyvärinen T, Hagman S, Ristola M, Sukki L, Veijula K, Kreutzer J, Kallio P, Narkilahti S (2019) Co-stimulation with IL-1? and TNF-? induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system. Sci Rep. 2019 Nov 15;9(1):16944. DOI: 10.1038/s41598-019-53414-9.
3.    Ylä-Outinen L, Tanskanen JMA, Kapucu FE, Hyysalo A, Hyttinen JAK, Narkilahti S. Advances in human stem cell-derived neuronal cell culturing and analysis. In Vitro Neuronal Networks - From Culturing Methods to Neuro-Technological Applications (Advances in Neurobiology, Vol. 22), M. Chiappalone, V. Pasquale, and M. Frega, Eds., Cham, Switzerland: Springer, 2019, pp. 299-329. https://doi.org/10.1007/978-3-030-11135-9_13
4.    Pekkanen-Mattila M, Häkli M, Pölönen R-P, Mansikkala T, Junnila A, Talvitie E, Koivisto JT, Kellomäki M, Aalto-Setälä K. Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Materials (Basel). 2019 Jun 3;12(11). pii: E1805. doi: 10.3390/ma12111805.
5.    Ryynänen T, Pelkonen A, Grigoras K, Ylivaara O.M.E, Hyvärinen T, Ahopelto J, Prunnila M, Narkilahti S, Lekkala J. Microelectrode array with transparent ALD TiN electrodes. Frontiers in Neuroscience. Accepted. (2019)
6.    Korkka I, Viheriälä T, Juuti?Uusitalo K, Uusitalo?Järvinen H, Skottman H, Hyttinen J, & Nymark, S (2019). Functional Voltage?Gated Calcium Channels Are Present in Human Embryonic Stem Cell?Derived Retinal Pigment Epithelium. Stem Cells Transl Med. 2019 Feb;8(2):179-193. doi: 10.1002/sctm.18-0026. Epub 2018 Nov 4.
7.    Kim J, Shah D, Potapov I, Latukka J, Aalto-Setälä K & Räsänen E. Scaling and correlation properties of RR and QT intervals at the cellular level. Scientific Reportsvolume 9, Article number: 3651 (2019)
8.    Johansson, J.K., Karema-Jokinen, V.I., Hakanen, S. et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol 17, 63 (2019). https://doi.org/10.1186/s12915-019-0681-
9.    Shah D, Virtanen L, Prajapati C, Kiamehr M, Gullmets J, West G, Kreutzer J, Pekkanen-Mattila M, Heliö T, Kallio P, Taimen P, Aalto-Setälä K. Modeling of LMNA-Related Dilated Cardiomyopathy Using Human Induced Pluripotent Stem Cells. Cells. 2019 Jun 15;8(6):594. doi: 10.3390/cells8060594. PMID: 31208058; PMCID: PMC6627421.

Ryynänen T, Toivanen M, Salminen T, Ylä-Outinen L, Narkilahti S, Lekkala J. Ion Beam Assisted E-Beam Deposited TiN Microelectrodes-Applied to Neuronal Cell Culture Medium Evaluation. Front Neurosci. 2018 Dec 4;12:882. doi: 10.3389/fnins.2018.00882. eCollection 2018.

Mäkinen ME, Ylä-Outinen L, Narkilahti S. GABA and Gap Junctions in the Development of Synchronized Activity in Human Pluripotent Stem Cell-Derived Neural Networks. Front Cell Neurosci. 2018 Mar 6;12:56. doi: 10.3389/fncel.2018.00056. eCollection 2018.

Pölönen R P,  Penttinen K, Swan H, Aalto-Setälä K. Antiarrhythmic Effects of Carvedilol and Flecainide in Cardiomyocytes Derived from Catecholaminergic Polymorphic Ventricular Tachycardia Patients. Stem Cells Int. 2018 Apr 12;2018:9109503. doi: 10.1155/2018/9109503. eCollection 2018.

Paci M, Pölönen R P, Cori D, Penttinen K, Aalto-Setälä K, Severi S, Hyttinen J. Automatic Optimization of an in Silico Model of Human iPSC Derived Cardiomyocytes Recapitulating Calcium Handling Abnormalities. Front. Physiol. 9:709. doi: 10.3389/fphys.2018.00709 (2018)

Prajapati C, Pölönen R P, Aalto-Setälä K. Simultaneous recordings of action potentials and calcium transients from human induced pluripotent stem cell derived cardiomyocytes. Biology Open 2018 : bio.035030 doi: 10.1242/bio.035030 Published 3 July 2018

Prajapati C, Ojala M, Aalto-Setälä K. Divergent effects of adrenaline in human induced pluripotent stem cell-derived cardiomyocytes obtained from hypertrophic cardiomyopathy. Disease models & mechanisms vol. 11,2 dmm032896. doi:10.1242/dmm.032896 (2018)

Ryynänen T, Pekkanen-Mattila M, Shah D, Kreutzer J, Kallio P, Lekkala J, Aalto-Setälä K. Microelectrode array for noninvasive analysis of cardiomyocytes at the single-cell level. Japanese Journal of Applied Physics 57, 117001 (2018)

Toivanen M, Pelkonen A, Mäkinen M, Ylä-Outinen L, Sukki L, Kallio P, Ristola M, Narkilahti S. Optimised PDMS Tunnel Devices on MEAs Increase the Probability of Detecting Electrical Activity from Human Stem Cell-Derived Neuronal Networks. Front Neurosci. 2017 Oct 31;11:606. doi: 10.3389/fnins.2017.00606. eCollection 2017.

Kreutzer J, Ylä-Outinen L, Mäki A-J, Ristola M, Narkilahti S, Kallio P. 2017. Cell culture chamber with gas supply for prolonged recording of human neuronal cells on microelectrode array. J Neurosci Methods. 2017 Mar 15;280:27-35.

Hyysalo A, Ristola M, Mäkinen M, Häyrynen S, Nykter M, Narkilahti S. 2017. Laminin alpha 5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro. Stem Cell Res. 2017 Oct;24:118-127

Korkka I, Johansson JK, Skottman H, Hyttinen J, Nymark S. Characterization of chloride channels in human embryonic stem cell derived retinal pigment epithelium. 2017 EMBEC and NBC 2017 – Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. Springer Verlag, p. 454-457 4 p. (IFMBE Proceedings; vol. 65)

Välkki IA, Lenk K, Mikkonen JE, Kapucu FE, Hyttinen JAK. Network-wide adaptive burst detection depicts neuronal activity with improved accuracy. 31 May 2017 In : Frontiers in Computational Neuroscience. 11, 40

Kapucu E, Tanskanen J, Christophe F, Mikkonen T, Hyttinen J. Evaluation of the effective and functional connectivity estimators for microelectrode array recordings during in vitro neuronal network maturation. 14 Jun 2017 EMBEC and NBC 2017 – Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. Springer Verlag, p. 1105-1108 (IFMBE Proceedings; vol. 65)

Böttrich M, Tanskanen JMA, Hyttinen JAK. Lead field theory provides a powerful tool for designing microelectrode array impedance measurements for biological cell detection and observation. 26 Jun 2017 In : Biomedical Engineering Online. 16, 1, 85

Kapucu E, Välkki IA, Christophe F, Tanskanen J, Johansson J, Mikkonen T, Hyttinen J. On electrophysiological signal complexity during biological neuronal network development and maturation. 15 Jul 2017 Proceedings of the 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

Skottman H, Muranen J, Lähdekorpi H, Pajula E, Mäkelä K, Koivusalo L, Koistinen J, Uusitalo H, Kaarniranta K, Juuti-Uusitalo K. An in vitro outer blood-retinal barrier model based on human embryonic stem cell derived retinal pigment epithelial cells and human endothelial cells. Exp Cell Res. 2017 Oct 1;359(1):101-111

Kuusela J, Larsson K, Shah D, Prajapati C, Aalto-Setälä K. Low extracellular potassium prolongs repolarization and evokes early afterdepolarization in human induced pluripotent stem cell-derived cardiomyocytes. Biol Open ;6(6)777-784, 2017.

Vuorenpää H, Penttinen K, Heinonen T, Pekkanen-Mattila M, Sarkanen JR, Ylikomi T, Aalto-Setälä K. Maturation of human pluripotent stem cell derived cardiomyocytes is improved in cardiovascular construct. Cytotechnology. 2017 Oct;69(5):785-800

Kapucu F, Mäkinen M, Tanskanen J, Ylä-Outinen L, Narkilahti S, Hyttinen J. Joint analysis of extracellular spike waveforms and neuronal network bursts. J Neurosci Methods. 2016 Feb 1;259:143-55.

Lenk K, Priwitzer B, Ylä-Outinen L, Tietz LH, Narkilahti S, Hyttinen JA. (2016) Simulation of developing human neuronal cell networks. Biomed Eng Online. 2016 Aug 30;15(1):105.

Abu Khamidakh AE, Dos Santos FC, Skottman H, Juuti-Uusitalo K, Hyttinen J. Semi-automatic Method for Ca2+ Imaging Data Analysis of Maturing Human Embryonic Stem Cells-Derived Retinal Pigment Epithelium. 2016 In : Annals of Biomedical Engineering. 44, 11, p. 1-13 13 p.

Kapucu FE, Mikkonen JE, Tanskanen JMA, Hyttinen JAK. Analyzing the feasibility of time correlated spectral entropy for the assessment of neuronal synchrony. 2016 2016 IEEE 38th Annual International Conference of the Engineering in Medicine and Biology Society (EMBC). IEEE

Alarautalahti V, Ragauskas S, Holme A, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S. Investigating retinal therapies using ex vivo retinal explants. 24 Jun 2016 In : Frontiers in Neuroscience.

Tanskanen JMA, Kapucu FE, Välkki I, Hyttinen JAK. Automatic objective thresholding to detect neuronal action potentials. 29 Aug 2016 Proceedings of 2016 24th European Signal Processing Conference (EUSIPCO). p. 662-666 5 p.

Lenk K, Räisänen E, Hyttinen JAK. Understanding the role of astrocytic GABA in simulated neural networks. 18 Oct 2016 2016 IEEE 38th Annual International Conference of the Engineering in Medicine and Biology Society (EMBC). IEEE, p. 6121-6124

Kapucu FE, Välkki I, Mikkonen JE, Leone C, Lenk K, Tanskanen JMA, Hyttinen JAK. Spectral Entropy Based Neuronal Network Synchronization Analysis Based on Microelectrode Array Measurements. 18 Oct 2016 In : Frontiers in Computational Neuroscience. 10, 112

Christophe F, Andalibi V, Lenk K, Mikkonen T, Hyttinen J. From in-silico Spiking Neural Networks to in-vitro Neural Networks. 20 Aug 2016 1 p. Proceedings of 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Orlando, Florida, USA

Tanskanen J, Kapucu FE, Hyttinen J. 2 Jul 2016. Automatic Objective Criterion for Setting Neuronal Action Potential Spike Detection Thresholds. 10th FENS Forum of Neuroscience – Copenhagen, Denmark

Välkki I, Kapucu FE, Johansson J, Lenk K, Hyttinen J. Characterizing Neuronal Network Burst Synchrony by Using Burst Signal. J. 24 Jun 2016, proceedings of the 10th International Meeting on Substrate-Integrated Electrode Arrays. Reutlingen, Germany. http://dx.doi.org/10.3389/conf.fnins.2016.93.00079

Alarautalahti V, Ragauskas S, Holme A, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S. Investigating retinal therapies using ex vivo retinal explants. 24 Jun 2016 In : Frontiers in Neuroscience.

Tanskanen J, Kapucu E, Välkki I, Lenk K, Hyttinen J. Objective Thresholding of MEA Data for Action Potential Detection. 24 Jun 2016. 10th International Meeting on Substrate-Integrated Electrode Arrays – Reutlingen, Germany.  http://dx.doi.org/10.3389/conf.fnins.2016.93.00028

Kapucu FE, Vornanen I, Tanskanen J, Christophe F, Hyttinen J. The Influence of Structural Changes and Population Interactions on the Entropy Based Synchronicity. 24 Jun 2016 2 p.10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany http://dx.doi.org/10.3389/conf.fnins.2016.93.00053

Paavola J, Väänänen H, Larsson K, Penttinen K, Toivonen L, Kontula K, Laine M, Aalto-Setälä K, Swan H, Viitasalo M. Slowed depolarization and irregular repolarization in catecholaminergic polymorphic ventricular tachycardia: a study from cellular Ca2+ transients and action potentials to clinical monophasic action potentials and electrocardiography. Europace ;18(10)1599-1607, 2016.

Kuusela J, Kim J, Räsänen E, Aalto-Setälä K. The Effects of Pharmacological Compounds on Beat Rate Variations in Human Long QT-Syndrome Cardiomyocytes. Stem Cell Rev ;12(6)698-707, 2016.

Ojala M, Prajapati C, Pölönen RP, Rajala K, Pekkanen-Mattila M, Rasku J, Larsson K, Aalto-Setälä K. Mutation-Specific Phenotypes in hiPSC-Derived Cardiomyocytes Carrying Either Myosin-Binding Protein C Or α-Tropomyosin Mutation for Hypertrophic Cardiomyopathy. Stem Cells Int ;2016.

Kuusela J, Kujala VJ, Kiviaho A, Ojala M, Swan H, Kontula K, Aalto-Setälä K. Effects of cardioactive drugs on human induced pluripotent stem cell derived long QT syndrome cardiomyocytes. Springerplus;2016.

The retinal pigment epithelium displays electrical excitability and lateral signal spreading.Ignatova I, Frolov R, Nymark S. BMC Biol. 2023 Apr 17;21(1):84. doi: 10.1186/s12915-023-01559-5. PMID: 37069561

Kapucu FE, Mikkonen JE, Tanskanen JMA, Hyttinen JAK. 2015. Quantification and automatized adaptive detection of in vivo and in vitro neuronal bursts based on signal complexity. 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). p. 4729-4732 4 p.

Laurila E, Ahola A, Hyttinen J, Aalto-Setälä K. Methods for in vitro functional analysis of iPSC derived cardiomyocytes — Special focus on analyzing the mechanical beating behaviour. 2015 In : Biochimica et Biophysica Acta: Molecular Cell Research.

Vainio I, Abu Khamidakh A, Paci M, Skottman H, Juuti-Uusitalo K, Hyttinen J, Nymark S. Computational Model of Ca2+ Wave Propagation in Human Retinal Pigment Epithelial ARPE-19 Cells. 2015 In : PLoS One. 0128434

Kapucu FE, Tanskanen JMA, Yuan Y, Hyttinen JAK. A fast stimulability screening protocol for neuronal cultures on microelectrode arrays. 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). p. 3440-3443 4 p

Juhola M, Penttinen K, Joutsijoki H, Varpa K, Saarikoski J, Rasku J, Siirtola H, Iltanen K, Laurikkala J, Hyyrö H, Hyttinen J, Aalto-Setälä K. Signal analysis and classification methods for the calcium transient data of stem cell-derived cardiomyocytes. 1 Jun 2015 In : Computers in Biology and Medicine. 61, p. 1-7 7 p.

Tanskanen JMA, Kapucu FE, Hyttinen JAK. On the threshold based neuronal spike detection, and an objective criterion for setting the threshold. 1 Jul 2015 International IEEE/EMBS Conference on Neural Engineering, NER. IEEE COMPUTER SOCIETY PRESS, p. 1016-1019 4 p.

Kiviaho AL, Ahola A, Larsson K, Penttinen K, Swan H, Pekkanen-Mattila M, Venäläinen H, Paavola K, Hyttinen J, Aalto-Setälä K. Distinct electrophysiological and mechanical beating phenotypes of long QT syndrome type 1-specific cardiomyocytes carrying different mutations. 1 Sep 2015 In : IJC Heart and Vasculature. 8, p. 19-31 13 p.

Penttinen K, Siirtola H, Àvalos-Salguero J, Vainio T, Juhola M, Aalto-Setälä K. Novel Analysis Software for Detecting and Classifying Ca2+ Transient Abnormalities in Stem Cell-Derived Cardiomyocytes. PLoS One 2015 ;10(8)e0135806.

Penttinen K, Swan H, Vanninen S, Paavola J, Lahtinen AM, Kontula K, Aalto-Setälä K. Antiarrhythmic Effects of Dantrolene in Patients with Catecholaminergic Polymorphic Ventricular Tachycardia and Replication of the Responses Using iPSC Models. PLoS One 2015 ;10(5)e0125366.

Abu Khamidakh A, Juuti-Uusitalo K, Caetano dos Santos F, Skottman H, Hyttinen J. Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation. 1 Oct 2015 In : Acta Ophthalmologica. 93, S225

Sorkio A, Porter PJ, Juuti-Uusitalo K, Meenan BJ, Skottman H, Burke GA. Atmospheric pressure plasma treatment increases the attachment and maturation of human pluripotent stem cell derived retinal pigment epithelial cells on biodegradable polymeric electrospun scaffolds. Tissue Eng Part A. 2015 Sep;21(17-18):2301-14.

Sorkio A, Vuorimaa-Laukkanen E, Hakola H, Liang H, Ujula T, Valle-Delgado JJ, Österberg M, Yliperttula M, Skottman H. Biomimetic Collagen I and IV double layer Langmuir-Schaeffer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells. Biomaterials. 2015 May;51:257-69. doi: 10.1016/j.biomaterials.2015.02.005. Epub 2015 Feb 20.

Ilmarinen I, Hiidenmaa H, Kööbi P, Nymark S, Sorkio A, Wang J-H, Stanzel BV, Thieltges F, Alajuuma P, Oksala O, Kataja M, Uusitalo H, Skottman H. Ultrathin Polyimide Membrane as Cell Carrier for Subretinal Transplantation of Human Embryonic Stem Cell Derived Retinal Pigment Epithelium. PLoS One. 2015; 10(11): e0143669. Published online 2015 Nov 25. doi:  10.1371/journal.pone.0143669

Sorkio A, Porter PJ, Juuti-Uusitalo K, Meenan BJ, Skottman H, Burke GA. Surface Modified Biodegradable Electrospun Membranes as a Carrier for Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells. Tissue Eng Part A. 2015 Sep;21(17-18):2301-14. doi: 10.1089/ten.TEA.2014.0640. Epub 2015 Jun 30.