Tampere Imaging Facility - light microscopy core
Introduction
Tampere Imaging Facility (TIF) - light microscopy core
The light microscopy facility offers state-of-the-art equipment for mesoscopic and high resolution bioimaging of tissues, small model organisms, biomaterials, tissue engineering products and advanced cell cultures both in 2D and 3D thus offering services for both life scientists and biomedical engineers.
Tampere Imaging Facility (TIF) is a light microscopy core unit located in the Arvo building on the Kauppi campus of the Tampere University. It offers state-of-the-art imaging solutions for life and material scientists. Facility includes several microscope systems for bioimaging of living and fixed specimens: spinning disk and laser scanning confocal microscopes, super-resolution structured illumination microscope, and widefield microscopes. In addition to the fluorescent microsopes, there are data processing and analysis stations available with deconvolution, visualization and image quantification possibilities.
Light microscopy core facility provides training for users, organizes imaging related courses and actively participates teaching at the Tampere University.
Microscopy and Image Analysis services (Sanni abroad in 2024)
Users now have the option to outsource their microscopy tasks or image analysis to our imaging specialists. We begin each project with a discussion regarding the specific needs and goals of the experimental setup, allowing us to tailor the service process to these requirements. After the planning stage, our specialist manages the entire project from start to finish, ensuring professional image acquisition and data quality. Instead of struggling with new devices or spending hours at the microscope, our users can focus on the results and their interpretation.
Through our imaging services, our objective is to offer a cost-effective solution for all imaging needs. Our billing system is very straightforward. The specialist bills the hours dedicated to the project plus the price of the device reservation.
Below are some examples of the services we provide:
- Planning and execution of experiments, such as live-cell, confocal or super-resolution imaging.
- Planning and execution of image processing and analysis tasks, such as deconvolution, channel alignment, drift correction, segmentation, colocalization, and more.
- ImageJ macros for batch processing.
- Measurements and data analysis tasks with ImageJ, Cell Profiler, Imaris, Python, RStudio, and more.
Would you like to collaborate with us? Contact us via our shared email (cofamicroscopy [at] lists.tuni.fi) or directly message the project specialist (sanni.eramies [at] tuni.fi).
Booking system
Publishing results obtained at the Tampere Imaging Facility
All the users of the Tampere Imaging Facility (TIF) services are obligated to acknowledge the facility and Biocenter Finland (BF) in publications, prefereably using the following phrase:
“The authors acknowledge the Biocenter Finland (BF) and Tampere Imaging Facility (TIF) for the service.”
This facility is partly supported by the Biocenter Finland technology platform network.
TUNICRIS
In addition, we request that you add Tampere Imaging Facility as Facilities/Equipment for that publication in TUNICRIS.
This allows us to monitor our contribution to the research community and helps us to gather funding to acquire new equipment.
Related research infrastructures at Tampere University
Superresolution Microscopes
Confocal Microscopes
Research Slide Scanners
Widefield Fluorescence Microscopes
Image Processing
Policies and Prices
User policy of Tampere Imaging Facility
The following document describes the user policy of Tampere Imaging Facility (TIF). Every user of the facility needs to read and carefully follow the practices.
Publishing results obtained at the Tampere Imaging Facility
All the users of the Tampere Imaging Facility (TIF) services are obligated to acknowledge the facility and Biocenter Finland (BF) in publications, prefereably using the following phrase:
“The authors acknowledge the Biocenter Finland (BF) and Tampere Imaging Facility (TIF) for the service.”
This facility is partly supported by the Biocenter Finland technology platform network.
TUNICRIS
In addition, we request that you add Tampere Imaging Facility as Facilities/Equipment for that publication in TUNICRIS
This allows us to monitor our contribution to the research community and helps us to gather funding to acquire new equipment.
General instrument usage rules
- No eating or drinking is allowed at the TIF laboratories.
- Lab coats must be worn in designated areas.
- Outdoor clothing is forbidden in TIF instrument areas. Shoe covers must be worn if clean indoor shoes are not used.
- Users must leave the instrument and its surroundings in clean condition.
- Users are not allowed to modify the instruments, replace objectives or other components without prior approval from the TIF staff.
- Users need to inform TIF staff in the case of instrument malfunction, breakage, or other problems.
- Don’t look to the laser beam with the remaining eye.
Access, training and booking
- Before first use. Contact TIF staff via cofamicroscopy [at] lists.tuni.fi (cofamicroscopy[at]lists[dot]tuni[dot]fi).
- Following the first contact, user training is arranged, preferably so that user has own samples ready.
- After user training, the user gets permission(s) to book the system(s) via Agendo: https://tuni.agendoscience.com/.
- User can book the system only for personal use (not for e.g., other not-trained group members).
Invoicing
- All instruments are charged per hour.
- Instrument hourly fees can be found from https://www.tuni.fi/en/research/tampere-imaging-facility-tif-light-microscopy-core-services#switcher-trigger-policies-and-prices.
- System usage will be invoiced biannually from the research groups.
Data storage and handling
- TIF is not responsible for data storage.
- User is responsible for removing the data from the instruments. TIF staff may delete over 2 months old data from the computer storage space without prior notice.
February 8th 2024
Prices
| COMPUTERS | PRICE/ HOUR |
| Image Analysis computer for deconvolution and other image processing (Agendo name: Image Analysis Deconvolution and Imaris) |
1,50 € |
| CONFOCAL MICROSCOPES | PRICE/ HOUR |
| Nikon A1R+ laser scanning confocal microscope (Agendo name: Nikon A1R with N-SIM) |
22,00 € |
| Nikon Eclipse FN1 upright spinning disk confocal microscope (Agendo name: Nikon FN1 Upright Fluorescence Microscope) |
8,00 € |
| Zeiss LSM 780 laser scanning confocal microscope (Agendo name: Zeiss LSM 780 LSCM) |
20,00 € |
| Zeiss LSM 800 laser scanning confocal microscope (Agendo name: Zeiss LSM 800 LSCM) |
15,00 € |
| SUPER-RESOLUTION MICROSCOPES | PRICE/ HOUR |
| Nikon N-SIM super-resolution microscope system (Agendo name: Nikon A1R with N-SIM) |
22,00 € |
| RESEARCH SLIDE SCANNERS | PRICE/ HOUR |
| Olympus SLIDEVIEW VS200 (Agendo name: Olympus VS200 Slide Scanner Microscope) |
10,00 € |
| WIDEFIELD FLUORESCENCE MICROSCOPES | PRICE/ HOUR |
| Nikon AZ100 multizoom upright fluorescence macroscope (Agendo name: Nikon AZ100 Fluorescence Macroscope) |
2,00 € |
| Nikon Eclipse Ti2 inverted fluorescence microscope (Agendo name: Nikon Eclipse Ti2 Fluorescence Microscope) |
5,00 € |
| Nikon Eclipse Ti Live inverted fluorescence microscope (Agendo name: Nikon Eclipse Ti Live Fluorescence Microscope) |
4,00 € |
| Olympus IX51 inverted fluorescence microscope (Agendo name: Olympus IX51 Fluorescence Microscope) |
1,00 € |
| Zeiss Axio Scope.A1 upright fluorescence microscope (Agendo name: Zeiss Axio Scope.A1 Fluorescence Microscope) |
1,00 € |
Pricing is valid for user groups from the Tampere University. Other academic or non-academic users are asked to inquire specific pricing.
Prices above are applicable from Monday to Friday 8 am to 4 pm. Discount of 50 % is applied for reservations on night time between 4 pm to 8 am and on weekends (there are no happy hour discounts for image analysis computer).
Tampere Imaging Facility reserves the right to price adjustments.
Tampere Imaging Facility - Publications (2014-2024)
2024
Komulainen T, Daymond P, Hietanen KE, Kaartinen IS, Järvinen TAH.
Myofibroblasts reside in the middle dermis of the keloids but do not predict the response to injection therapies: a double-blinded, randomized, controlled trial.
Front Med (Lausanne). 2024 Mar 1;11:1293028. doi: 10.3389/fmed.2024.1293028.
PMID: 38495113
Abraham BG, Haikarainen T, Vuorio J, Girych M, Virtanen AT, Kurttila A, Karathanasis C, Heilemann M, Sharma V, Vattulainen I, Silvennoinen O.
Molecular basis of JAK2 activation in erythropoietin receptor and pathogenic JAK2 signaling.
Sci Adv. 2024 Mar 8;10(10):eadl2097. doi: 10.1126/sciadv.adl2097.
PMID: 38457493
Grönroos P, Mörö A, Puistola P, Hopia K, Huuskonen M, Viheriälä T, Ilmarinen T, Skottman H.
Bioprinting of human pluripotent stem cell derived corneal endothelial cells with hydrazone crosslinked hyaluronic acid bioink.
Stem Cell Res Ther. 2024 Mar 14;15(1):81. doi: 10.1186/s13287-024-03672-w.
PMID: 38486306
Szczodra A, Houaoui A, Salminen T, Hannula M, Gobbo VA, Ghanavati S, Miettinen S, Massera J.
Pore graded borosilicate bioactive glass scaffolds: in vitro dissolution and cytocompatibility.
J Mater Sci Mater Med. 2024 Mar 20;35(1):17. doi: 10.1007/s10856-024-06791-1.
PMID: 38507150
Kärki T, Koskimäki S, Guenther C, Pirhonen J, Rajakylä K, Tojkander S.
Mechanosensitive TRPV4 channel guides maturation and organization of the bilayered mammary epithelium.
Sci Rep. 2024 Mar 21;14(1):6774. doi: 10.1038/s41598-024-57346-x.
PMID: 38514727
Puistola P, Kethiri A, Nurminen A, Turkki J, Hopia K, Miettinen S, Mörö A, Skottman H.
Cornea-Specific Human Adipose Stem Cell-Derived Extracellular Matrix for Corneal Stroma Tissue Engineering.
ACS Appl Mater Interfaces. 2024 Apr 3;16(13):15761-15772. doi: 10.1021/acsami.3c17803.
PMID: 38513048
2023
Hyväri L, Vanhatupa S, Ojansivu M, Kelloniemi M, Pakarinen TK, Hupa L, Miettinen S.
Heat Shock Protein 27 Is Involved in the Bioactive Glass Induced Osteogenic Response of Human Mesenchymal Stem Cells.
Cells. 2023 Jan 5;12(2):224. doi: 10.3390/cells12020224.
PMID: 36672159
Alexanova A, Raitoharju E, Valtonen J, Aalto-Setälä K, Viiri LE.
Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism.
Sci Rep. 2023 Jan 30;13(1):1706. doi: 10.1038/s41598-023-28981-7.
PMID: 36717592
Hellinen L, Hongisto H, Ramsay E, Kaarniranta K, Vellonen KS, Skottman H, Ruponen M.
Comparison of barrier properties of outer blood-retinal barrier models - Human stem cell-based models as a novel tool for ocular drug discovery.
Eur J Pharm Biopharm. 2023 Mar;184:181-188. doi: 10.1016/j.ejpb.2023.01.026.
PMID: 36740104
Karema-Jokinen V, Koskela A, Hytti M, Hongisto H, Viheriälä T, Liukkonen M, Torsti T, Skottman H, Kauppinen A, Nymark S, Kaarniranta K.
Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients.
J Biol Chem. 2023 May 1:104770. doi: 10.1016/j.jbc.2023.104770.
PMID: 37137441
Mahmoud M, Juntunen M, Adnan A, Kummola L, Junttila IS, Kelloniemi M, Tyrväinen T, Huhtala H, Abd El Fattah AI, Amr K, El Erian AM, Patrikoski M, Miettinen S.
Immunomodulatory Functions of Adipose Mesenchymal Stromal/Stem Cell Derived From Donors With Type 2 Diabetes and Obesity on CD4 T Cells.
Stem Cells. 2023 May 15;41(5):505-519. doi: 10.1093/stmcls/sxad021.
PMID: 36945068
Hammarén MM, Luukinen H, Sillanpää A, Remans K, Lapouge K, Custódio T, Löw C, Myllymäki H, Montonen T, Seeger M, Robertson J, Nyman TA, Savijoki K, Parikka M.
In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies.
mSystems. 2023 May 15:e0107322. doi: 10.1128/msystems.01073-22.
PMID: 37184670
Sartoneva R, Lyyra I, Juusela M, Sharma V, Huhtala H, Massera J, Kellomäki M, Miettinen S.
In vitro biocompatibility of polylactide and polybutylene succinate blends for urethral tissue engineering.
J Biomed Mater Res B Appl Biomater. 2023 May 18. doi: 10.1002/jbm.b.35268.
PMID: 37199480
Valtonen J, Prajapati C, Cherian RM, Vanninen S, Ojala M, Leivo K, Heliö T, Koskenvuo J, Aalto-Setälä K.
The Junctophilin-2 Mutation p.(Thr161Lys) Is Associated with Hypertrophic Cardiomyopathy Using Patient-Specific iPS Cardiomyocytes and Demonstrates Prolonged Action Potential and Increased Arrhythmogenicity.
Biomedicines. 2023 May 27;11(6):1558. doi: 10.3390/biomedicines11061558.
PMID: 37371654
Isosaari L, Vuorenpää H, Yrjänäinen A, Kapucu FE, Kelloniemi M, Pakarinen TK, Miettinen S, Narkilahti S.
Simultaneous induction of vasculature and neuronal network formation on a chip reveals a dynamic interrelationship between cell types.
Cell Commun Signal. 2023 Jun 14;21(1):132. doi: 10.1186/s12964-023-01159-4.
PMID: 37316873
Mäntylä E, Montonen T, Azzari L, Mattola S, Hannula M, Vihinen-Ranta M, Hyttinen J, Vippola M, Foi A, Nymark S, Ihalainen TO.
Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina.
Mol Biol Cell. 2023 Jun 21:mbcE22090448. doi: 10.1091/mbc.E22-09-0448.
PMID: 37342871
Kerslake R, Belay B, Panfilov S, Hall M, Kyrou I, Randeva HS, Hyttinen J, Karteris E, Sisu C.
Transcriptional Landscape of 3D vs. 2D Ovarian Cancer Cell Models.
Cancers (Basel). 2023 Jun 26;15(13):3350. doi: 10.3390/cancers15133350.
PMID: 37444459
Isola J, Mäki M, Hils M, Pasternack R, Viiri K, Dotsenko V, Montonen T, Zimmermann T, Mohrbacher R, Greinwald R, Schuppan D.
The Oral Transglutaminase 2 Inhibitor ZED1227 Accumulates in the Villous Enterocytes in Celiac Disease Patients during Gluten Challenge and Drug Treatment.
Int J Mol Sci. 2023 Jun 28;24(13):10815. doi: 10.3390/ijms241310815.
PMID: 37445994
Danielsson BE, George Abraham B, Mäntylä E, Cabe JI, Mayer CR, Rekonen A, Ek F, Conway DE, Ihalainen TO.
Nuclear lamina strain states revealed by intermolecular force biosensor.
Nat Commun. 2023 Jun 30;14(1):3867. doi: 10.1038/s41467-023-39563-6.
PMID: 37391402
Tervonen A, Korpela S, Nymark S, Hyttinen J, Ihalainen TO.
The Effect of Substrate Stiffness on Elastic Force Transmission in the Epithelial Monolayers over Short Timescales.
Cell Mol Bioeng. 2023 Jul 13;16(5-6):475-495. doi: 10.1007/s12195-023-00772-0.
PMID: 38099211
Ilmarinen T, Vattulainen M, Kandhavelu J, Bremond-Gignac D, Aberdam D, Skottman H.
Production and limbal lineage commitment of aniridia patient-derived induced pluripotent stem cells.
Stem Cells. 2023 Aug 26:sxad067. doi: 10.1093/stmcls/sxad067.
PMID: 37632794
Suominen S, Hyypijev T, Venäläinen M, Yrjänäinen A, Vuorenpää H, Lehti-Polojärvi M, Räsänen M, Seppänen A, Hyttinen J, Miettinen S, Aalto-Setälä K, Viiri LE.
Improvements in Maturity and Stability of 3D iPSC-Derived Hepatocyte-like Cell Cultures.
Cells. 2023 Sep 27;12(19):2368. doi: 10.3390/cells12192368.
PMID: 37830581
Belay B, Mäntylä E, Maibohm C, Silvestre OF, Hyttinen J, Nieder JB, Ihalainen TO.
Substrate microtopographies induce cellular alignment and affect nuclear force transduction.
J Mech Behav Biomed Mater. 2023 Oct;146:106069. doi: 10.1016/j.jmbbm.2023.106069.
PMID: 37586175
Sartoneva R, Paakinaho K, Hannula M, Kuismanen K, Huhtala H, Hyttinen J, Miettinen S.
Ascorbic Acid 2-Phosphate Releasing Supercritically Foamed Porous Poly-L-Lactide-Co-ε-Caprolactone Scaffold Enhances the Collagen Production of Human Vaginal Stromal Cells: A New Approach for Vaginal Tissue Engineering.
Tissue Eng Regen Med. 2023 Oct 31. doi: 10.1007/s13770-023-00603-3.
PMID: 37907765
Kauppila M, Mörö A, Valle-Delgado JJ, Ihalainen T, Sukki L, Puistola P, Kallio P, Ilmarinen T, Österberg M, Skottman H.
Toward Corneal Limbus In Vitro Model: Regulation of hPSC-LSC Phenotype by Matrix Stiffness and Topography During Cell Differentiation Process.
Adv Healthc Mater. 2023 Nov;12(29):e2301396. doi: 10.1002/adhm.202301396.
PMID: 37449943
Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytönen VP, Howarth M.
Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion.
J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 10.1021/jacs.3c07827.
PMID: 38104267
Peussa H, Fedele C, Tran H, Marttinen M, Fadjukov J, Mäntylä E, Priimägi A, Nymark S, Ihalainen TO.
Light-Induced Nanoscale Deformation in Azobenzene Thin Film Triggers Rapid Intracellular Ca2+ Increase via Mechanosensitive Cation Channels.
Adv Sci (Weinh). 2023 Dec;10(35):e2206190. doi: 10.1002/advs.202206190.
PMID: 37946608
Terzioglu M, Veeroja K, Montonen T, Ihalainen TO, Salminen TS, Bénit P, Rustin P, Chang YT, Nagai T, Jacobs HT.
Mitochondrial temperature homeostasis resists external metabolic stresses.
Elife. 2023 Dec 11;12:RP89232. doi: 10.7554/eLife.89232.
PMID: 38079477
Zapilko V, Moisio S, Parikka M, Heinäniemi M, Lohi O.
Generation of a Zebrafish Knock-In Model Recapitulating Childhood ETV6::RUNX1-Positive B-Cell Precursor Acute Lymphoblastic Leukemia.
Cancers (Basel). 2023 Dec 13;15(24):5821. doi: 10.3390/cancers15245821.
PMID: 38136366
Puistola P, Miettinen S, Skottman H, Mörö A.
Novel strategy for multi-material 3D bioprinting of human stem cell based corneal stroma with heterogenous design.
Mater Today Bio. 2023 Dec 22;24:100924. doi: 10.1016/j.mtbio.2023.100924.
PMID: 38226015
2022
Saari J, Siddique F, Korpela S, Mäntylä E, Ihalainen TO, Kaukinen K, Aalto-Setälä K, Lindfors K, Juuti-Uusitalo K.
Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells.
Int J Mol Sci. 2022 Jan 24;23(3):1312. doi: 10.3390/ijms23031312.
PMID: 35163236
Viheriälä T, Hongisto H, Sorvari J, Skottman H, Nymark S, Ilmarinen T.
Cell maturation influences the ability of hESC-RPE to tolerate cellular stress.
Stem Cell Res Ther. 2022 Jan 24;13(1):30. doi: 10.1186/s13287-022-02712-7.
PMID: 35073969
Mykuliak A, Yrjänäinen A, Mäki AJ, Gebraad A, Lampela E, Kääriäinen M, Pakarinen TK, Kallio P, Miettinen S, Vuorenpää H.
Vasculogenic Potency of Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells Results in Differing Vascular Network Phenotypes in a Microfluidic Chip.
Front Bioeng Biotechnol. 2022 Feb 8;10:764237. doi: 10.3389/fbioe.2022.764237.
PMID: 35211462
Häkli M, Jäntti S, Joki T, Sukki L, Tornberg K, Aalto-Setälä K, Kallio P, Pekkanen-Mattila M, Narkilahti S.
Human Neurons Form Axon-Mediated Functional Connections with Human Cardiomyocytes in Compartmentalized Microfluidic Chip.
Int J Mol Sci. 2022 Mar 15;23(6):3148. doi: 10.3390/ijms23063148.
PMID: 35328569
Gaballah M, Penttinen K, Kreutzer J, Mäki AJ, Kallio P, Aalto-Setälä K.
Cardiac Ischemia On-a-Chip: Antiarrhythmic Effect of Levosimendan on Ischemic Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Cells. 2022 Mar 19;11(6):1045. doi: 10.3390/cells11061045.
PMID: 35326497
Fadjukov J, Wienbar S, Hakanen S, Aho V, Vihinen-Ranta M, Ihalainen TO, Schwartz GW, Nymark S.
Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium.
J Gen Physiol. 2022 Apr 4;154(4):e202112916. doi: 10.1085/jgp.202112916.
PMID: 35275193
Mzezewa R, Lotila J, Kiiski H, Vinogradov A, Kapucu FE, Peltola J, Hagman S, Narkilahti S.
A kainic acid-induced seizure model in human pluripotent stem cell-derived cortical neurons for studying the role of IL-6 in the functional activity.
Stem Cell Res. 2022 Apr;60:102665. doi: 10.1016/j.scr.2022.102665.
PMID: 35091307
Gebraad A, Ohlsbom R, Miettinen JJ, Emeh P, Pakarinen TK, Manninen M, Eskelinen A, Kuismanen K, Slipicevic A, Lehmann F, Nupponen NN, Heckman CA, Miettinen S.
Growth Response and Differentiation of Bone Marrow-Derived Mesenchymal Stem/Stromal Cells in the Presence of Novel Multiple Myeloma Drug Melflufen.
Cells. 2022 May 7;11(9):1574. doi: 10.3390/cells11091574.
PMID: 35563880
Peussa H, Kreutzer J, Mäntylä E, Mäki AJ, Nymark S, Kallio P, Ihalainen TO.
Pneumatic equiaxial compression device for mechanical manipulation of epithelial cell packing and physiology.
PLoS One. 2022 Jun 3;17(6):e0268570. doi: 10.1371/journal.pone.0268570.
PMID: 35657824
Samanta S, Ylä-Outinen L, Rangasami VK, Narkilahti S, Oommen OP.
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain mimetic 3D scaffold.
Acta Biomater. 2022 Jul 15;147:439. doi: 10.1016/j.actbio.2022.05.025.
PMID: 35597769
Korkka I, Skottman H, Nymark S.
Heterogeneity of Potassium Channels in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium.
Stem Cells Transl Med. 2022 Jul 20;11(7):753-766. doi: 10.1093/stcltm/szac029.
PMID: 35639962
Azizi L, Varela L, Turkki P, Mykuliak VV, Korpela S, Ihalainen TO, Church J, Hytönen VP, Goult BT.
Talin variant P229S compromises integrin activation and associates with multifaceted clinical symptoms.
Hum Mol Genet. 2022 Jul 21:ddac163. doi: 10.1093/hmg/ddac163.
PMID: 35861643
Maria Cherian R, Prajapati C, Penttinen K, Häkli M, Koivisto JT, Pekkanen-Mattila M, Aalto-Setälä K.
Fluorescent hiPSC-derived MYH6-mScarlet cardiomyocytes for real-time tracking, imaging, and cardiotoxicity assays.
Cell Biol Toxicol. 2022 Jul 23. doi: 10.1007/s10565-022-09742-0.
PMID: 35870039
Lotila J, Hyvärinen T, Skottman H, Airas L, Narkilahti S, Hagman S.
Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient.
Stem Cell Res. 2022 Aug;63:102865. doi: 10.1016/j.scr.2022.102865.
PMID: 35843021
Koivisto M, Tolvanen TA, Toimela T, Miinalainen I, Kiviaho A, Kesseli J, Nykter M, Eklund L, Heinonen T.
Functional human cell-based vascularised cardiac tissue model for biomedical research and testing.
Sci Rep. 2022 Aug 5;12(1):13459. doi: 10.1038/s41598-022-17498-0.
PMID: 35931748
Karttunen J, Heiskanen M, Joki T, Hyysalo A, Navarro-Ferrandis V, Miettinen S, Narkilahti S, Pitkänen A.
Effect of cell culture media on extracellular vesicle secretion from mesenchymal stromal cells and neurons.
Eur J Cell Biol. 2022 Sep-Nov;101(4):151270. doi: 10.1016/j.ejcb.2022.151270.
PMID: 35987046
Räsänen N, Harju V, Joki T, Narkilahti S.
Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions.
Development. 2022 Oct 15;149(20):dev200012. doi: 10.1242/dev.200012.
PMID: 35929583
Tornberg K, Välimäki H, Valaskivi S, Mäki AJ, Jokinen M, Kreutzer J, Kallio P.
Compartmentalized organ-on-a-chip structure for spatiotemporal control of oxygen microenvironments.
Biomed Microdevices. 2022 Oct 21;24(4):34. doi: 10.1007/s10544-022-00634-y.
PMID: 36269438
Gaertner K, Michell C, Tapanainen R, Goffart S, Saari S, Soininmäki M, Dufour E, Pohjoismäki JLO.
Molecular phenotyping uncovers differences in basic housekeeping functions among closely related species of hares (Lepus spp., Lagomorpha: Leporidae).
Mol Ecol. 2022 Nov 1. doi: 10.1111/mec.16755.
PMID: 36320183
Kukkonen K, Autio-Kimura B, Rauhala H, Kesseli J, Nykter M, Latonen L, Visakorpi T.
Nonmalignant AR-positive prostate epithelial cells and cancer cells respond differently to androgen.
Endocr Relat Cancer. 2022 Nov 7;29(12):717-733. doi: 10.1530/ERC-22-0108.
PMID: 36219867
Gering C, Párraga J, Vuorenpää H, Botero L, Miettinen S, Kellomäki M.
Bioactivated gellan gum hydrogels affect cellular rearrangement and cell response in vascular co-culture and subcutaneous implant models.
Biomater Adv. 2022 Dec;143:213185. doi: 10.1016/j.bioadv.2022.213185.
PMID: 36371972
Häkli M, Kreutzer J, Mäki AJ, Välimäki H, Cherian RM, Kallio P, Aalto-Setälä K, Pekkanen-Mattila M.
Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation.
Stem Cells Int. 2022 Dec 19;2022:9438281. doi: 10.1155/2022/9438281.
PMID: 36579142
Mörö A, Samanta S, Honkamäki L, Rangasami VK, Puistola P, Kauppila M, Narkilahti S, Miettinen S, Oommen O, Skottman H.
Hyaluronic acid based next generation bioink for 3D bioprinting of human stem cell derived corneal stromal model with innervation.
Biofabrication. 2022 Dec 29;15(1). doi: 10.1088/1758-5090/acab34.
PMID: 36579828
2021
Tan GSW, Liu Z, Ilmarinen T, Barathi VA, Chee CK, Lingam G, Su X, Stanzel BV.
Hints for Gentle Submacular Injection in Non-Human Primates Based on Intraoperative OCT Guidance.
Transl Vis Sci Technol. 2021 Jan 7;10(1):10. doi: 10.1167/tvst.10.1.10.
PMID: 33510949
Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytönen VP.
Cancer associated talin point mutations disorganise cell adhesion and migration.
Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4.
PMID: 33431906
Viheriälä T, Sorvari J, Ihalainen TO, Mörö A, Grönroos P, Schlie-Wolter S, Chichkov B, Skottman H, Nymark S, Ilmarinen T.
Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE.
Sci Rep. 2021 Jan 13;11(1):933. doi: 10.1038/s41598-020-79638-8.
PMID: 33441679
Grönroos P, Ilmarinen T, Skottman H.
Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions.
Cells. 2021 Feb 5;10(2):331. doi: 10.3390/cells10020331.
PMID: 33562615
Honkamäki L, Joki T, Grigoryev NA, Levon K, Ylä-Outinen L, Narkilahti S.
Novel method to produce a layered 3D scaffold for human pluripotent stem cell-derived neuronal cells.
J Neurosci Methods. 2021 Feb 15;350:109043. doi: 10.1016/j.jneumeth.2020.109043.
PMID: 33345946
Häkli M, Kreutzer J, Mäki AJ, Välimäki H, Lappi H, Huhtala H, Kallio P, Aalto-Setälä K, Pekkanen-Mattila M.
Human induced pluripotent stem cell-based platform for modeling cardiac ischemia.
Sci Rep. 2021 Feb 18;11(1):4153. doi: 10.1038/s41598-021-83740-w.
PMID: 33603154
Anton-Sales I, Koivusalo L, Skottman H, Laromaine A, Roig A.
Limbal Stem Cells on Bacterial Nanocellulose Carriers for Ocular Surface Regeneration.
Small. 2021 Mar;17(10):e2003937. doi: 10.1002/smll.202003937.
PMID: 33586332
Prajapati C, Ojala M, Lappi H, Aalto-Setälä K, Pekkanen-Mattila M.
Electrophysiological evaluation of human induced pluripotent stem cell-derived cardiomyocytes obtained by different methods.
Stem Cell Res. 2021 Mar;51:102176. doi: 10.1016/j.scr.2021.102176.
PMID: 33485184
Eerola SK, Kohvakka A, Tammela TLJ, Koskinen PJ, Latonen L, Visakorpi T.
Expression and ERG regulation of PIM kinases in prostate cancer.
Cancer Med. 2021 May;10(10):3427-3436. doi: 10.1002/cam4.3893.
PMID: 33932111
George JJ, Oittinen M, Martin-Diaz L, Zapilko V, Iqbal S, Rintakangas T, Arrojo Martins FT, Niskanen H, Katajisto P, Kaikkonen MU, Viiri K.
Polycomb Repressive Complex 2 Regulates Genes Necessary for Intestinal Microfold Cell (M Cell) Development.
Cell Mol Gastroenterol Hepatol. 2021;12(3):873-889. doi: 10.1016/j.jcmgh.2021.05.014.
PMID: 34058415
Hytti M, Korhonen E, Hongisto H, Kaarniranta K, Skottman H, Kauppinen A.
Differential Expression of Inflammasome-Related Genes in Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells with or without History of Age-Related Macular Degeneration.
Int J Mol Sci. 2021 Jun 24;22(13):6800. doi: 10.3390/ijms22136800.
PMID: 34202702
Savijoki K, Myllymäki H, Luukinen H, Paulamäki L, Vanha-Aho LM, Svorjova A, Miettinen I, Fallarero A, Ihalainen TO, Yli-Kauhaluoma J, Nyman TA, Parikka M.
Surface-Shaving Proteomics of Mycobacterium marinum Identifies Biofilm Subtype-Specific Changes Affecting Virulence, Tolerance, and Persistence.
mSystems. 2021 Jun 29;6(3):e0050021. doi: 10.1128/mSystems.00500-21.
PMID: 34156290
Liu Z, Ilmarinen T, Tan GSW, Hongisto H, Wong EYM, Tsai ASH, Al-Nawaiseh S, Holder GE, Su X, Barathi VA, Skottman H, Stanzel BV.
Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model.
Stem Cell Res Ther. 2021 Jul 27;12(1):423. doi: 10.1186/s13287-021-02395-6.
PMID: 34315534
Azizi L, Turkki P, Huynh N, Massera JM, Hytönen VP.
Surface Modification of Bioactive Glass Promotes Cell Attachment and Spreading.
ACS Omega. 2021 Aug 25;6(35):22635-22642. doi: 10.1021/acsomega.1c02669.
PMID: 34514235
George JJ, Martin-Diaz L, Ojanen MJT, Gasa R, Pesu M, Viiri K.
PRC2 Regulated Atoh8 Is a Regulator of Intestinal Microfold Cell (M Cell) Differentiation.
Int J Mol Sci. 2021 Aug 28;22(17):9355. doi: 10.3390/ijms22179355.
PMID: 34502262
Hyunh NB, Palma CSD, Rahikainen R, Mishra A, Azizi L, Verne E, Ferraris S, Hytönen VP, Sanches Ribeiro A, Massera J.
Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption.
ACS Biomater Sci Eng. 2021 Sep 13;7(9):4483-4493. doi: 10.1021/acsbiomaterials.1c00735.
PMID: 34382772
Mäntylä E, Ihalainen TO.
Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells.
Sci Rep. 2021 Sep 16;11(1):18520. doi: 10.1038/s41598-021-97900-5.
PMID: 34531455
Juntunen M, Heinonen S, Huhtala H, Rissanen A, Kaprio J, Kuismanen K, Pietiläinen KH, Miettinen S, Patrikoski M.
Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs.
Stem Cell Res Ther. 2021 Sep 26;12(1):516. doi: 10.1186/s13287-021-02587-0.
PMID: 34565451
Leppiniemi J, Mutahir Z, Dulebo A, Mikkonen P, Nuopponen M, Turkki P, Hytönen VP.
Avidin-Conjugated Nanofibrillar Cellulose Hydrogel Functionalized with Biotinylated Fibronectin and Vitronectin Promotes 3D Culture of Fibroblasts.
Biomacromolecules. 2021 Oct 11;22(10):4122-4137. doi: 10.1021/acs.biomac.1c00579.
PMID: 34542997
Coentro JQ, May U, Prince S, Zwaagstra J, Ritvos O, Järvinen TAH, Zeugolis DI.
Adapting the Scar-in-a-Jar to Skin Fibrosis and Screening Traditional and Contemporary Anti-Fibrotic Therapies.
Front Bioeng Biotechnol. 2021 Oct 26;9:756399. doi: 10.3389/fbioe.2021.756399.
PMID: 34765594
Ahtiainen A, Genocchi B, Tanskanen JMA, Barros MT, Hyttinen JAK, Lenk K.
Astrocytes Exhibit a Protective Role in Neuronal Firing Patterns under Chemically Induced Seizures in Neuron-Astrocyte Co-Cultures.
Int J Mol Sci. 2021 Nov 25;22(23):12770. doi: 10.3390/ijms222312770.
PMID: 34884577
Buchacher T, Honkimaa A, Välikangas T, Lietzén N, Hirvonen MK, Laiho JE, Sioofy-Khojine AB, Eskelinen EL, Hyöty H, Elo LL, Lahesmaa R.
Persistent coxsackievirus B1 infection triggers extensive changes in the transcriptome of human pancreatic ductal cells.
iScience. 2021 Dec 18;25(1):103653. doi: 10.1016/j.isci.2021.103653.
PMID: 35024587
Vattulainen M, Ilmarinen T, Viheriälä T, Jokinen V, Skottman H.
Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5+ and ∆Np63α+ cells with limbal cell characteristics and high wound healing capacity.
Stem Cell Res Ther. 2021 Dec 20;12(1):609. doi: 10.1186/s13287-021-02673-3.
PMID: 34930437
Järvelä-Stölting M, Vesala L, Maasdorp MK, Ciantar J, Rämet M, Valanne S.
Proteasome α6 Subunit Negatively Regulates the JAK/STAT Pathway and Blood Cell Activation in Drosophila melanogaster.
Front Immunol. 2021 Dec 22;12:729631. doi: 10.3389/fimmu.2021.729631.
PMID: 35003057
Hernández-Pérez S, Runsala M, Šuštar V, Mattila PK.
Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight.
Methods Mol Biol. 2021;2304:173-191. doi: 10.1007/978-1-0716-1402-0_9.
PMID: 34028717
2020
Calejo MT, Saari J, Vuorenpää H, Vuorimaa-Laukkanen E, Kallio P, Aalto-Setälä K, Miettinen S, Skottman H, Kellomäki M, Juuti-Uusitalo K.
Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films.
Acta Biomater. 2020 Jan 1;101:327-343. doi: 10.1016/j.actbio.2019.11.002.
PMID: 31711900
Halonen HT, Ihalainen TO, Hyväri L, Miettinen S, Hyttinen JAK.
Cell adhesion and culture medium dependent changes in the high frequency mechanical vibration induced proliferation, osteogenesis, and intracellular organization of human adipose stem cells.
J Mech Behav Biomed Mater. 2020 Jan;101:103419. doi: 10.1016/j.jmbbm.2019.103419.
PMID: 31518945
Harjula SE, Saralahti AK, Ojanen MJT, Rantapero T, Uusi-Mäkelä MIE, Nykter M, Lohi O, Parikka M, Rämet M.
Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio).
Dev Comp Immunol. 2020 Feb;103:103523. doi: 10.1016/j.dci.2019.103523.
PMID: 31626817
Kreutzer J, Viehrig M, Pölönen RP, Zhao F, Ojala M, Aalto-Setälä K, Kallio P.
Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes.
Biomech Model Mechanobiol. 2020 Feb;19(1):291-303. doi: 10.1007/s10237-019-01211-8.
PMID: 31444593
Shah D, Prajapati C, Penttinen K, Cherian RM, Koivumäki JT, Alexanova A, Hyttinen J, Aalto-Setälä K.
hiPSC-Derived Cardiomyocyte Model of LQT2 Syndrome Derived from Asymptomatic and Symptomatic Mutation Carriers Reproduces Clinical Differences in Aggregates but Not in Single Cells.
Cells. 2020 May 7;9(5):1153. doi: 10.3390/cells9051153.
PMID: 32392813
Välimäki H, Hyvärinen T, Leivo J, Iftikhar H, Pekkanen-Mattila M, Rajan DK, Verho J, Kreutzer J, Ryynänen T, Pirhonen J, Aalto-Setälä K, Kallio P, Narkilahti S, Lekkala J.
Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity.
Biomed Microdevices. 2020 Jun 3;22(2):41. doi: 10.1007/s10544-020-00495-3.
PMID: 32494857
Kohvakka A, Sattari M, Shcherban A, Annala M, Urbanucci A, Kesseli J, Tammela TLJ, Kivinummi K, Latonen L, Nykter M, Visakorpi T.
AR and ERG drive the expression of prostate cancer specific long noncoding RNAs.
Oncogene. 2020 Jul;39(30):5241-5251. doi: 10.1038/s41388-020-1365-6.
PMID: 32555329
Pemmari T, Ivanova L, May U, Lingasamy P, Tobi A, Pasternack A, Prince S, Ritvos O, Makkapati S, Teesalu T, Cairo MS, Järvinen TAH, Liao Y.
Exposed CendR Domain in Homing Peptide Yields Skin-Targeted Therapeutic in Epidermolysis Bullosa.
Mol Ther. 2020 Aug 5;28(8):1833-1845. doi: 10.1016/j.ymthe.2020.05.017.
PMID: 32497513
Fedele C, Mäntylä E, Belardi B, Hamkins-Indik T, Cavalli S, Netti PA, Fletcher DA, Nymark S, Priimagi A, Ihalainen TO.
Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells.
Sci Rep. 2020 Sep 18;10(1):15329. doi: 10.1038/s41598-020-71567-w.
PMID: 32948792
Hyväri L, Vanhatupa S, Halonen HT, Kääriäinen M, Miettinen S.
Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells.
Stem Cells Int. 2020 Sep 22;2020:8853541. doi: 10.1155/2020/8853541.
PMID: 33029150
Hongisto H, Dewing JM, Christensen DR, Scott J, Cree AJ, Nättinen J, Määttä J, Jylhä A, Aapola U, Uusitalo H, Kaarniranta K, Ratnayaka JA, Skottman H, Lotery AJ.
In vitro stem cell modelling demonstrates a proof-of-concept for excess functional mutant TIMP3 as the cause of Sorsby fundus dystrophy.
J Pathol. 2020 Oct;252(2):138-150. doi: 10.1002/path.5506.
PMID: 32666594
Tamminen I, Lehto K, Hannula M, Ojansivu M, Johansson L, Kellomäki M, Miettinen S, Aula A, Ihalainen T, Hyttinen J.
A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samples.
Commun Biol. 2020 Oct 2;3(1):548. doi: 10.1038/s42003-020-01273-w.
PMID: 33009501
Juntunen M, Hagman S, Moisan A, Narkilahti S, Miettinen S.
In Vitro Oxygen-Glucose Deprivation-Induced Stroke Models with Human Neuroblastoma Cell- and Induced Pluripotent Stem Cell-Derived Neurons.
Stem Cells Int. 2020 Oct 29;2020:8841026. doi: 10.1155/2020/8841026.
PMID: 33178286
Honkimaa A, Kimura B, Sioofy-Khojine AB, Lin J, Laiho J, Oikarinen S, Hyöty H.
Genetic Adaptation of Coxsackievirus B1 during Persistent Infection in Pancreatic Cells.
Microorganisms. 2020 Nov 15;8(11):1790. doi: 10.3390/microorganisms8111790.
PMID: 33203081
Pelkonen A, Mzezewa R, Sukki L, Ryynänen T, Kreutzer J, Hyvärinen T, Vinogradov A, Aarnos L, Lekkala J, Kallio P, Narkilahti S.
A modular brain-on-a-chip for modelling epileptic seizures with functionally connected human neuronal networks.
Biosens Bioelectron. 2020 Nov 15;168:112553. doi: 10.1016/j.bios.2020.112553.
PMID: 32877779
Jäntti T, Luhtala S, Mäenpää J, Staff S.
Characterization of immunoreactivity with whole-slide imaging and digital analysis in high-grade serous ovarian cancer.
Tumour Biol. 2020 Nov;42(11):1010428320971404. doi: 10.1177/1010428320971404.
PMID: 33169632
Rasheed A, Azizi L, Turkki P, Janka M, Hytönen VP, Tuukkanen S.
Extrusion-Based Bioprinting of Multilayered Nanocellulose Constructs for Cell Cultivation Using In Situ Freezing and Preprint CaCl2 Cross-Linking.
ACS Omega. 2020 Dec 30;6(1):569-578. doi: 10.1021/acsomega.0c05036.
PMID: 33458509
2019
Karvinen J, Ihalainen TO, Calejo MT, Jönkkäri I, Kellomäki M.
Characterization of the microstructure of hydrazone crosslinked polysaccharide-based hydrogels through rheological and diffusion studies.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:1056-1066. doi: 10.1016/j.msec.2018.10.048.
PMID: 30423686
Valanne S, Salminen TS, Järvelä-Stölting M, Vesala L, Rämet M.
Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster.
PLoS Pathog. 2019 Jan 11;15(1):e1007504. doi: 10.1371/journal.ppat.1007504.
PMID: 30633769
Korkka I, Viheriälä T, Juuti-Uusitalo K, Uusitalo-Järvinen H, Skottman H, Hyttinen J, Nymark S.
Functional Voltage-Gated Calcium Channels Are Present in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium.
Stem Cells Transl Med. 2019 Feb;8(2):179-193. doi: 10.1002/sctm.18-0026.
PMID: 30394009
Viiri LE, Rantapero T, Kiamehr M, Alexanova A, Oittinen M, Viiri K, Niskanen H, Nykter M, Kaikkonen MU, Aalto-Setälä K.
Extensive reprogramming of the nascent transcriptome during iPSC to hepatocyte differentiation.
Sci Rep. 2019 Mar 5;9(1):3562. doi: 10.1038/s41598-019-39215-0.
PMID: 30837492
González de Cózar JM, Gerards M, Teeri E, George J, Dufour E, Jacobs HT, Jõers P.
RNase H1 promotes replication fork progression through oppositely transcribed regions of Drosophila mitochondrial DNA.
J Biol Chem. 2019 Mar 22;294(12):4331-4344. doi: 10.1074/jbc.RA118.007015.
PMID: 30635398
Aspatwar A, Tolvanen MEE, Schneider HP, Becker HM, Narkilahti S, Parkkila S, Deitmer JW.
Catalytically-inactive carbonic anhydrase-related proteins enhance transport of lactate by MCT1.
FEBS Open Bio. 2019 Apr 29. doi: 10.1002/2211-5463.12647.
PMID: 31033227
Rahikainen R, Öhman T, Turkki P, Varjosalo M, Hytönen VP.
Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling.
J Cell Sci. 2019 Apr 3;132(7). pii: jcs226514. doi: 10.1242/jcs.226514.
PMID: 30837291
Hammarén HM, Virtanen AT, Abraham BG, Peussa H, Hubbard SR, Silvennoinen O.
Janus kinase 2 activation mechanisms revealed by analysis of suppressing mutations.
J Allergy Clin Immunol. 2019 Apr;143(4):1549-1559.e6. doi: 10.1016/j.jaci.2018.07.022.
PMID: 30092288
Alarautalahti V, Ragauskas S, Hakkarainen JJ, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S.
Viability of Mouse Retinal Explant Cultures Assessed by Preservation of Functionality and Morphology.
Invest Ophthalmol Vis Sci. 2019 May 1;60(6):1914-1927. doi: 10.1167/iovs.18-25156.
PMID: 31042799
Pekkanen-Mattila M, Häkli M, Pölönen RP, Mansikkala T, Junnila A, Talvitie E, Koivisto JT, Kellomäki M, Aalto-Setälä K.
Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Materials (Basel). 2019 Jun 3;12(11). pii: E1805. doi: 10.3390/ma12111805.
PMID: 31163704
Koivisto JT, Gering C, Karvinen J, Maria Cherian R, Belay B, Hyttinen J, Aalto-Setälä K, Kellomäki M, Parraga J.
Mechanically Biomimetic Gelatin-Gellan Gum Hydrogels for 3D Culture of Beating Human Cardiomyocytes.
ACS Appl Mater Interfaces. 2019 Jun 12;11(23):20589-20602. doi: 10.1021/acsami.8b22343.
PMID: 31120238
Vuornos K, Ojansivu M, Koivisto JT, Häkkänen H, Belay B, Montonen T, Huhtala H, Kääriäinen M, Hupa L, Kellomäki M, Hyttinen J, Ihalainen JA, Miettinen S.
Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:905-918. doi: 10.1016/j.msec.2019.02.035.
PMID: 30889765
Aspatwar A, Tolvanen MEE, Schneider HP, Becker HM, Narkilahti S, Parkkila S, Deitmer JW.
Catalytically inactive carbonic anhydrase-related proteins enhance transport of lactate by MCT1.
FEBS Open Bio. 2019 Jul;9(7):1204-1211. doi: 10.1002/2211-5463.12647.
PMID: 31033227
Ylä-Outinen L, Harju V, Joki T, Koivisto JT, Karvinen J, Kellomäki M, Narkilahti S.
Screening of Hydrogels for Human Pluripotent Stem Cell-Derived Neural Cells: Hyaluronan-Polyvinyl Alcohol-Collagen-Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold.
Macromol Biosci. 2019 Jul;19(7):e1900096. doi: 10.1002/mabi.201900096.
PMID: 31173471
Kiamehr M, Klettner A, Richert E, Koskela A, Koistinen A, Skottman H, Kaarniranta K, Aalto-Setälä K, Juuti-Uusitalo K.
Compromised Barrier Function in Human Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells from Type 2 Diabetic Patients.
Int J Mol Sci. 2019 Aug 1;20(15). pii: E3773. doi: 10.3390/ijms20153773.
PMID: 31375001
Vattulainen M, Ilmarinen T, Koivusalo L, Viiri K, Hongisto H, Skottman H.
Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential.
Stem Cell Res Ther. 2019 Aug 5;10(1):236. doi: 10.1186/s13287-019-1354-2.
PMID:31383008
Johansson JK, Karema-Jokinen VI, Hakanen S, Jylhä A, Uusitalo H, Vihinen-Ranta M, Skottman H, Ihalainen TO, Nymark S.
Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium.
BMC Biol. 2019 Aug 15;17(1):63. doi: 10.1186/s12915-019-0681-1.
PMID: 31412898
Gering C, Koivisto JT, Parraga J, Leppiniemi J, Vuornos K, Hytönen VP, Miettinen S, Kellomäki M.
Design of modular gellan gum hydrogel functionalized with avidin and biotinylated adhesive ligands for cell culture applications.
PLoS One. 2019 Aug 30;14(8):e0221931. doi: 10.1371/journal.pone.0221931.
PMID: 31469884
Lietzén N, Hirvonen K, Honkimaa A, Buchacher T, Laiho JE, Oikarinen S, Mazur MA, Flodström-Tullberg M, Dufour E, Sioofy-Khojine AB, Hyöty H, Lahesmaa R.
Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells.
iScience. 2019 Sep 27;19:340-357. doi: 10.1016/j.isci.2019.07.040.
PMID: 31404834
Bahhir D, Yalgin C, Ots L, Järvinen S, George J, Naudí A, Krama T, Krams I, Tamm M, Andjelković A, Dufour E, González de Cózar JM, Gerards M, Parhiala M, Pamplona R, Jacobs HT, Jõers P.
Manipulating mtDNA in vivo reprograms metabolism via novel response mechanisms.
PLoS Genet. 2019 Oct 4;15(10):e1008410. doi: 10.1371/journal.pgen.1008410.
PMID: 31584940
Harjula SE, Saralahti AK, Ojanen MJT, Rantapero T, Uusi-Mäkelä MIE, Nykter M, Lohi O, Parikka M, Rämet M.
Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio).
Dev Comp Immunol. 2019 Oct 15;103:103523. doi: 10.1016/j.dci.2019.103523.
PMID: 31626817
Calejo MT, Saari J, Vuorenpää H, Vuorimaa-Laukkanen E, Kallio P, Aalto-Setälä K, Miettinen S, Skottman H, Kellomäki M, Juuti-Uusitalo K.
Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films.
Acta Biomater. 2019 Nov 8. pii: S1742-7061(19)30742-1. doi: 10.1016/j.actbio.2019.11.002.
PMID: 31711900
Hyvärinen T, Hyysalo A, Kapucu F.E, Aarnos L, Vinogradov A, Eglen S.J, Ylä-Outinen L, Narkilahti S.
Co-stimulation with IL-1β and TNF-α induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system.
Sci Rep. 2019 Nov 15;9(1):16944. doi: 10.1038/s41598-019-53414-9.
PMID: 31729450
Hyvärinen T, Hyysalo A, Kapucu FE, Aarnos L, Vinogradov A, Eglen SJ, Ylä-Outinen L, Narkilahti S.
Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures.
Sci Rep. 2019 Nov 20;9(1):17125. doi: 10.1038/s41598-019-53647-8.
PMID: 31748598
Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytönen VP, Howarth M.
Approaching infinite affinity through engineering of peptide-protein interaction.
Proc Natl Acad Sci U S A. 2019 Dec 10;116(52):26523-33. doi: 10.1073/pnas.1909653116.
PMID: 31822621
Koivusalo L, Kauppila M, Samanta S, Parihar VS, Ilmarinen T, Miettinen S, Oommen OP, Skottman H.
Tissue adhesive hyaluronic acid hydrogels for sutureless stem cell delivery and regeneration of corneal epithelium and stroma.
Biomaterials. 2019 Dec;225:119516. doi: 10.1016/j.biomaterials.2019.119516.
PMID: 31574405
2018
Saarinen NVV, Laiho JE, Richardson SJ, Zeissler M, Stone VM, Marjomäki V, Kantoluoto T, Horwitz MS, Sioofy-Khojine A, Honkimaa A, Hankaniemi MM, Flodström-Tullberg M, Hyöty H, Hytönen VP, Laitinen OH.
A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses.
Sci Rep. 2018 Jan 8;8(1):33. doi: 10.1038/s41598-017-18495-4.
PMID: 29311608
Gerards M, Cannino G, de Cózar JMG, Jacobs HT.
Intracellular vesicle trafficking plays an essential role in mitochondrial quality control.
Mol Biol Cell. 2018 Jan 17. pii: mbc.E17-10-0619. doi: 10.1091/mbc.E17-10-0619.
PMID: 29343549
Ojansivu M, Wang X, Hyväri L, Kellomäki M, Hupa L, Vanhatupa S, Miettinen S.
Bioactive glass induced osteogenic differentiation of human adipose stem cells is dependent on cell attachment mechanism and mitogen-activated protein kinases.
Eur Cell Mater. 2018 Jan; 35:54-72. doi: 10.22203/eCM.v035a05.
PMID: 29383695
Vähätupa M, Cordova ZM, Barker H, Aittomäki S, Uusitalo H, Järvinen TAH, Pesu M, Uusitalo-Järvinen H.
Furin deficiency in myeloid cells leads to attenuated revascularization in a mouse-model of oxygen-induced retinopathy.
Exp Eye Res. 2018 Jan;166:160-167. doi: 10.1016/j.exer.2017.10.013.
PMID: 29031855
Aspatwar A, Haapanen S, Parkkila S.
An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.
Metabolites. 2018 Mar 13;8(1). pii: E22. doi: 10.3390/metabo8010022.
PMID: 29534024
Vuorinen EM, Rajala NK, Ihalainen TO, Kallioniemi A.
Depletion of nuclear import protein karyopherin alpha 7 (KPNA7) induces mitotic defects and deformation of nuclei in cancer cells.
BMC Cancer. 2018 Mar 27;18(1):325. doi: 10.1186/s12885-018-4261-5.
PMID: 29580221
Paavilainen T, Pelkonen A, Mäkinen ME, Peltola M, Huhtala H, Fayuk D, Narkilahti S.
Effect of prolonged differentiation on functional maturation of human pluripotent stem cell-derived neuronal cultures.
Stem Cell Res. 2018 Mar;27:151-161. doi: 10.1016/j.scr.2018.01.018.
PMID: 29414606
Koivusalo L, Karvinen J, Sorsa E, Jönkkäri I, Väliaho J, Kallio P, Ilmarinen T, Miettinen S, Skottman H, Kellomäki M.
Hydrazone crosslinked hyaluronan-based hydrogels for therapeutic delivery of adipose stem cells to treat corneal defects.
Mater Sci Eng C Mater Biol Appl. 2018 Apr 1;85:68-78. doi: 10.1016/j.msec.2017.12.013.
PMID: 29407158
Ojansivu M, Johansson L, Vanhatupa S, Tamminen I, Hannula M, Hyttinen J, Kellomäki M, Miettinen S.
Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis.
Stem Cells Int. 2018 May 7;2018:5928935. doi: 10.1155/2018/5928935.
PMID: 29853915
Vähätupa M, Nättinen J, Jylhä A, Aapola U, Kataja M, Kööbi P, Järvinen TAH, Uusitalo H, Uusitalo-Järvinen H.
SWATH-MS Proteomic Analysis of Oxygen-Induced Retinopathy Reveals Novel Potential Therapeutic Targets.
Invest Ophthalmol Vis Sci. 2018 Jul 2;59(8):3294-3306. doi: 10.1167/iovs.18-23831.
PMID: 30025079
Prajapati C, Pölönen RP, Aalto-Setälä K.
Simultaneous recordings of action potentials and calcium transients from human induced pluripotent stem cell derived cardiomyocytes.
Biol Open. 2018 Jul 16;7(7). pii: bio035030. doi: 10.1242/bio.035030.
PMID: 29970475
Abu Khamidakh AE, Rodriguez-Martinez A, Kaarniranta K, Kallioniemi A, Skottman H, Hyttinen J, Juuti-Uusitalo K.
Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage.
Biomed Eng Online. 2018 Jul 31;17(1):102. doi: 10.1186/s12938-018-0535-z.
PMID: 30064430
Sorkio A, Koch L, Koivusalo L, Deiwick A, Miettinen S, Chichkov B, Skottman H.
Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks.
Biomaterials. 2018 Jul;171:57-71. doi: 10.1016/j.biomaterials.2018.04.034.
PMID: 29684677
Hammarén HM, Virtanen AT, Abraham BG, Peussa H, Hubbard SR, Silvennoinen O.
Janus kinase 2 activation mechanisms revealed by analysis of suppressing mutations.
J Allergy Clin Immunol. 2018 Aug 6. pii: S0091-6749(18)31129-1. doi: 10.1016/j.jaci.2018.07.022.
PMID: 30092288
Sartoneva R, Kuismanen K, Juntunen M, Karjalainen S, Hannula M, Kyllönen L, Hyttinen J, Huhtala H, Paakinaho K, Miettinen S.
Porous poly-l-lactide-co-ɛ-caprolactone scaffold: a novel biomaterial for vaginal tissue engineering.
R Soc Open Sci. 2018 Aug 15;5(8):180811. doi: 10.1098/rsos.180811.
PMID: 30225072
Ojansivu M, Mishra A, Vanhatupa S, Juntunen M, Larionova A, Massera J, Miettinen S.
The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells.
PLoS One. 2018 Aug 28;13(8):e0202740. doi: 10.1371/journal.pone.0202740.
PMID: 30153295
Hagman S, Mäkinen A, Ylä-Outinen L, Huhtala H, Elovaara I, Narkilahti S.
Effects of inflammatory cytokines IFN-γ, TNF-α and IL-6 on the viability and functionality of human pluripotent stem cell-derived neural cells.
J Neuroimmunol. 2018 Aug 29. pii: S0165-5728(17)30480-0. doi: 10.1016/j.jneuroim.2018.07.010.
PMID: 30195439
Hyväri L, Ojansivu M, Juntunen M, Kartasalo K, Miettinen S, Vanhatupa S.
Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages.
Stem Cells Int. 2018 Sep 12;2018:2190657. doi: 10.1155/2018/2190657.
PMID: 30275837
Hongisto H, Vattulainen M, Ilmarinen T, Mikhailova A, Skottman H.
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells.
J Vis Exp. 2018 Oct 24;(140). doi: 10.3791/58279.
PMID: 30417867
Andjelković A, Mordas A, Bruinsma L, Ketola A, Cannino G, Giordano L, Dhandapani PK, Szibor M, Dufour E, Jacobs HT.
Expression of the Alternative Oxidase Influences Jun N-Terminal Kinase Signaling and Cell Migration.
Mol Cell Biol. 2018 Nov 28;38(24). pii: e00110-18. doi: 10.1128/MCB.00110-18.
PMID: 30224521
Ryynänen T, Toivanen M, Salminen T, Ylä-Outinen L, Narkilahti S, Lekkala J.
Ion Beam Assisted E-Beam Deposited TiN Microelectrodes-Applied to Neuronal Cell Culture Medium Evaluation.
Front Neurosci. 2018 Dec 4;12:882. doi: 10.3389/fnins.2018.00882.
PMID: 30568570
Ilmarinen T, Thieltges F, Hongisto H, Juuti-Uusitalo K, Koistinen A, Kaarniranta K, Brinken R, Braun N, Holz FG, Skottman H, Stanzel BV.
Survival and functionality of xeno-free human embryonic stem cell-derived retinal pigment epithelial cells on polyester substrate after transplantation in rabbits.
Acta Ophthalmol. 2018 Dec 28. doi: 10.1111/aos.14004.
PMID: 30593729
2017
Kumar M, Rahikainen R, Unruh D, Hytönen VP, Delbrück C, Sindelar R, Renz F.
Mixture of PLA-PEG and biotinylated albumin enables immobilization of avidins on electrospun fibers.
J Biomed Mater Res A. 2017 Feb;105(2):356-362. doi: 10.1002/jbm.a.35920.
PMID: 27684712
Kreutzer J, Ylä-Outinen L, Mäki AJ, Ristola M, Narkilahti S, Kallio P.
Cell culture chamber with gas supply for prolonged recording of human neuronal cells on microelectrode array.
J Neurosci Methods. 2017 Mar 15;280:27-35. doi: 10.1016/j.jneumeth.2017.01.019.
PMID: 28161299
Koivisto JT, Joki T, Parraga JE, Pääkkönen R, Ylä-Outinen L, Salonen L, Jönkkäri I, Peltola M, Ihalainen TO, Narkilahti S, Kellomäki M.
Bioamine-crosslinked gellan gum hydrogel for neural tissue engineering.
Biomed Mater. 2017 Mar 24;12(2):025014. doi: 10.1088/1748-605X/aa62b0.
PMID: 28233757
Juuti-Uusitalo K, Koskela A, Kivinen N, Viiri J, Hyttinen JMT, Reinisalo M, Koistinen A, Uusitalo H, Sinha D, Skottman H, Kaarniranta K.
Autophagy Regulates Proteasome Inhibitor-Induced Pigmentation in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.
Int J Mol Sci. 2017 May 19;18(5). pii: E1089. doi: 10.3390/ijms18051089.
PMID: 28534814
Oksala NKJ, Seppälä I, Rahikainen R, Mäkelä KM, Raitoharju E, Illig T, Klopp N, Kholova I, Laaksonen R, Karhunen PJ, Hytönen VP, Lehtimäki T.
Synergistic Expression of Histone Deacetylase 9 and Matrix Metalloproteinase 12 in M4 Macrophages in Advanced Carotid Plaques.
Eur J Vasc Endovasc Surg. 2017 May;53(5):632-640. doi: 10.1016/j.ejvs.2017.02.014.
PMID: 28343758
Calejo MT, Ilmarinen T, Vuorimaa-Laukkanen E, Talvitie E, Hakola HM, Skottman H, Kellomäki M.
Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering.
Acta Biomater. 2017 May;54:138-149. doi: 10.1016/j.actbio.2017.02.035.
PMID: 28223209
Rahikainen R, von Essen M, Schaefer M, Qi L, Azizi L, Kelly C, Ihalainen TO, Wehrle-Haller B, Bastmeyer M, Huang C, Hytönen VP.
Mechanical stability of talin rod controls cell migration and substrate sensing.
Sci Rep. 2017 Jun 15;7(1):3571. doi: 10.1038/s41598-017-03335-2.
PMID: 2862017
Saari S, Andjelković A, Garcia GS, Jacobs HT, Oliveira MT.
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster.
BMC Dev Biol. 2017 Jul 3;17(1):9. doi: 10.1186/s12861-017-0151-3.
PMID: 28673232
Leppiniemi J, Lahtinen P, Paajanen A, Mahlberg R, Metsä-Kortelainen S, Pinomaa T, Pajari H, Vikholm-Lundin I, Pursula P, Hytönen VP.
3D-Printable Bioactivated Nanocellulose-Alginate Hydrogels.
ACS Appl Mater Interfaces. 2017 Jul 5;9(26):21959-21970. doi: 10.1021/acsami.7b02756.
PMID: 28598154
Leivo J, Virjula S, Vanhatupa S, Kartasalo K, Kreutzer J, Miettinen S, Kallio P.
A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture.
J R Soc Interface. 2017 Jul;14(132). pii: 20170318. doi: 10.1098/rsif.2017.0318.
PMID: 28747398
Hyysalo A, Ristola M, Joki T, Honkanen M, Vippola M, Narkilahti S.
Aligned Poly(ε-caprolactone) Nanofibers Guide the Orientation and Migration of Human Pluripotent Stem Cell-Derived Neurons, Astrocytes, and Oligodendrocyte Precursor Cells In Vitro.
Macromol Biosci. 2017 Jul;17(7). doi: 10.1002/mabi.201600517.
PMID: 28296144
Skottman H, Muranen J, Lähdekorpi H, Pajula E, Mäkelä K, Koivusalo L, Koistinen A, Uusitalo H, Kaarniranta K, Juuti-Uusitalo K.
Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells.
Exp Cell Res. 2017 Aug 8. pii: S0014-4827(17)30415-9. doi: 10.1016/j.yexcr.2017.08.004.
PMID: 28800962
Turunen S, Joki T, Hiltunen ML, Ihalainen TO, Narkilahti S, Kellomäki M.
Direct Laser Writing of Tubular Microtowers for 3D Culture of Human Pluripotent Stem Cell-Derived Neuronal Cells.
ACS Appl Mater Interfaces. 2017 Aug 9;9(31):25717-25730. doi: 10.1021/acsami.7b05536.
PMID: 28697300
Virjula S, Zhao F, Leivo J, Vanhatupa S, Kreutzer J, Vaughan TJ, Honkala AM, Viehrig M, Mullen CA, Kallio P, McNamara LM, Miettinen S.
The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells.
J Mech Behav Biomed Mater. 2017 Aug;72:38-48. doi: 10.1016/j.jmbbm.2017.04.016.
PMID: 28448920
Kiamehr M, Viiri LE, Vihervaara T, Koistinen KM, Hilvo M, Ekroos K, Käkelä R, Aalto-Setälä K.
Lipidomic profiling of patient-specific iPSC-derived hepatocyte-like cells.
Dis Model Mech. 2017 Sep 1;10(9):1141-1153. doi: 10.1242/dmm.030841.
PMID: 28733363
Hyysalo A, Ristola M, Mäkinen ME, Häyrynen S, Nykter M, Narkilahti S.
Laminin α5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro.
Stem Cell Res. 2017 Sep 12;24:118-127. doi: 10.1016/j.scr.2017.09.002.
PMID: 28926760
Vuorenpää H, Penttinen K, Heinonen T, Pekkanen-Mattila M, Sarkanen JR, Ylikomi T, Aalto-Setälä K.
Maturation of human pluripotent stem cell derived cardiomyocytes is improved in cardiovascular construct.
Cytotechnology. 2017 Oct;69(5):785-800. doi: 10.1007/s10616-017-0088-1.
PMID: 28397099
Sorkio A, Haimi S, Verdoold V, Juuti-Uusitalo K, Grijpma D, Skottman H.
Poly(trimethylene carbonate) as an elastic biodegradable film for human embryonic stem cell-derived retinal pigment epithelial cells.
J Tissue Eng Regen Med. 2017 Nov;11(11):3134-3144. doi: 10.1002/term.2221.
PMID:28052579
Hongisto H, Ilmarinen T, Vattulainen M, Mikhailova A, Skottman H.
Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method.
Stem Cell Res Ther. 2017 Dec 29;8(1):291. doi: 10.1186/s13287-017-0738-4.
PMID:29284513
2016
Kemppainen E, George J, Garipler G, Tuomela T, Kiviranta E, Soga T, Dunn CD, Jacobs HT.
Mitochondrial Dysfunction Plus High-Sugar Diet Provokes a Metabolic Crisis That Inhibits Growth.
PLoS One. 2016 Mar 8;11(3):e0151421. doi: 10.1371/journal.pone.0151421.
PMID: 26954062
Vuornos K, Björninen M, Talvitie E, Paakinaho K, Kellomäki M, Huhtala H, Miettinen S, Seppänen-Kaijansinkko R, Haimi S.
Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds Is a Potential Approach for Tendon Tissue Engineering.
Tissue Eng Part A. 2016 Mar;22(5-6):513-23. doi: 10.1089/ten.tea.2015.0276.
PMID: 26919401
Calejo MT, Ilmarinen T, Jongprasitkul H, Skottman H, Kellomäki M.
Honeycomb porous films as permeable scaffold materials for human embryonic stem cell-derived retinal pigment epithelium.
J Biomed Mater Res A. 2016 Jul;104(7):1646-56. doi: 10.1002/jbm.a.35690.
PMID: 26914698
Anderl I, Vesala L, Ihalainen TO, Vanha-Aho LM, Andó I, Rämet M, Hultmark D.
Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection.
PLoS Pathog. 2016 Jul 14;12(7):e1005746. doi: 10.1371/journal.ppat.1005746.
PMID: 27414410
Hynninen V, Vuori L, Hannula M, Tapio K, Lahtonen K, Isoniemi T, Lehtonen E, Hirsimäki M, Toppari JJ, Valden M, Hytönen VP.
Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology.
Sci Rep. 2016 Jul 6;6:29324. doi: 10.1038/srep29324.
PMID: 27381834
Lenk K, Priwitzer B, Ylä-Outinen L, Tietz LH, Narkilahti S, Hyttinen JA.
Simulation of developing human neuronal cell networks.
Biomed Eng Online. 2016 Aug 30;15(1):105. doi: 10.1186/s12938-016-0226-6.
PMID: 27576323
Andjelković A, Kemppainen KK, Jacobs HT.
Ligand-Bound GeneSwitch Causes Developmental Aberrations in Drosophila that Are Alleviated by the Alternative Oxidase.
G3 (Bethesda). 2016 Sep 8;6(9):2839-46. doi: 10.1534/g3.116.030882.
PMID: 27412986
von Essen M, Rahikainen R, Oksala N, Raitoharju E, Seppälä I, Mennander A, Sioris T, Kholová I, Klopp N, Illig T, Karhunen PJ, Kähönen M, Lehtimäki T, Hytönen VP.
Talin and vinculin are downregulated in atherosclerotic plaque; Tampere Vascular Study.
Atherosclerosis. 2016 Dec;255:43-53. doi: 10.1016/j.atherosclerosis.2016.10.031.
PMID: 27816808
2015
Faia-Torres AB, Goren T, Ihalainen TO, Guimond-Lischer S, Charnley M, Rottmar M, Maniura-Weber K, Spencer ND, Reis RL, Textor M, Neves NM.
Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: a gradient study.
ACS Appl Mater Interfaces. 2015 Feb 4;7(4):2367-75. doi: 10.1021/am506951c.
PMID: 25513839
Vanha-Aho LM, Anderl I, Vesala L, Hultmark D, Valanne S, Rämet M.
Edin Expression in the Fat Body Is Required in the Defense Against Parasitic Wasps in Drosophila melanogaster.
PLoS Pathog. 2015 May 12;11(5):e1004895. doi: 10.1371/journal.ppat.1004895.
PMID: 25965263
Ribeiro C, Pärssinen J, Sencadas V, Correia V, Miettinen S, Hytönen VP, Lanceros-Méndez S.
Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells.
J Biomed Mater Res A. 2015 Jun;103(6):2172-5. doi: 10.1002/jbm.a.35368.
PMID: 25370596
Syrjänen L, Valanne S, Kuuslahti M, Tuomela T, Sriram A, Sanz A, Jacobs HT, Rämet M, Parkkila S.
β carbonic anhydrase is required for female fertility in Drosophila melanogaster.
Front Zool. 2015 Aug 22;12:19. doi: 10.1186/s12983-015-0111-3.
PMID: 26300950
Oksala N, Pärssinen J, Seppälä I, Klopp N, Illig T, Laaksonen R, Levula M, Raitoharju E, Kholova I, Sioris T, Kähönen M, Lehtimäki T, Hytönen VP.
Kindlin 3 (FERMT3) is associated with unstable atherosclerotic plaques, anti-inflammatory type II macrophages and upregulation of beta-2 integrins in all major arterial beds.
Atherosclerosis. 2015 Sep;242(1):145-54. doi: 10.1016/j.atherosclerosis.2015.06.058.
PMID: 26188538
Koho T, Ihalainen TO, Stark M, Uusi-Kerttula H, Wieneke R, Rahikainen R, Blazevic V, Marjomäki V, Tampé R, Kulomaa MS, Hytönen VP.
His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.
Eur J Pharm Biopharm. 2015 Oct;96:22-31. doi: 10.1016/j.ejpb.2015.07.002.
PMID: 26170162
Ihalainen TO, Aires L, Herzog FA, Schwartlander R, Moeller J, Vogel V.
Differential basal-to-apical accessibility of lamin A/C epitopes in the nuclear lamina regulated by changes in cytoskeletal tension.
Nat Mater. 2015 Dec;14(12):1252-61. doi: 10.1038/nmat4389.
PMID: 26301768
2014
Rajala N, Gerhold JM, Martinsson P, Klymov A, Spelbrink JN.
Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication.
Nucleic Acids Res. 2014 Jan;42(2):952-67. doi: 10.1093/nar/gkt988.
PMID: 24163258
Kemppainen KK, Rinne J, Sriram A, Lakanmaa M, Zeb A, Tuomela T, Popplestone A, Singh S, Sanz A, Rustin P, Jacobs HT.
Expression of alternative oxidase in Drosophila ameliorates diverse phenotypes due to cytochrome oxidase deficiency.
Hum Mol Genet. 2014 Apr 15;23(8):2078-93. doi: 10.1093/hmg/ddt601.
PMID: 24293544
Vartiainen S, Chen S, George J, Tuomela T, Luoto KR, O'Dell KM, Jacobs HT.
Phenotypic rescue of a Drosophila model of mitochondrial ANT1 disease.
Dis Model Mech. 2014 Jun;7(6):635-48. doi: 10.1242/dmm.016527.
PMID: 24812436
Fukuoh A, Cannino G, Gerards M, Buckley S, Kazancioglu S, Scialo F, Lihavainen E, Ribeiro A, Dufour E, Jacobs HT.
Screen for mitochondrial DNA copy number maintenance genes reveals essential role for ATP synthase.
Mol Syst Biol. 2014 Jun 21;10:734. doi: 10.15252/msb.20145117.
PMID: 24952591
User Support
Imaging Systems:
Support’s shared email address:
cofamicroscopy [at] lists.tuni.fi
Laboratory Assistant:
Eija Hannuksela (on maternity leave)
Room: Arvo D443
Imaging Specialists:
Sanni Erämies (abroad in 2024)
Room: Arvo D223
Outi Paloheimo
Room: Arvo D224
Project Manager:
Jenni Karttunen
Room: Arvo D223
Management:
Teemu Ihalainen, Ph.D., Adjunct Professor (docent)
Telephone: +358 503187202
Room: Arvo D337