Lignocellulosic biomass, derived from plant materials, is a promising renewable resource for the sustainable production of chemicals and materials. However, its conversion is hindered by the presence of toxic compounds and the heterogeneous and recalcitrant nature of its components, particularly the lignin fraction. Changshuo Liu’s work demonstrated that A. baylyi ADP1 can efficiently detoxify common lignocellulose hydrolysate inhibitors, such as furan aldehydes, organic acids, and aromatic compounds, improving the feasibility of using this carbon and energy rich feedstock directly in microbial processes.
To demonstrate the applicability of A. baylyi ADP1 for lignocellulose hydrolysate detoxification, Changshuo Liu and his collaborators constructed a synthetic microbial consortium of A. baylyi ADP1 and the industrial yeast, Saccharomyces cerevisiae used for lactic acid production. The co-culture approach improved lactic acid productivity and enabled more efficient and comprehensive carbon upgrading. In addition, Liu developed a growth-coupled enzyme screening system in A. baylyi ADP1 to identify efficient decarboxylases that catalyze key steps in the conversion of lignin-derived aromatic compounds into cis,cis-muconic acid, a precursor for bio-based plastics.
His research addresses two major challenges in the microbial conversion of lignocellulosic biomass: the toxicity of biomass-derived inhibitors and the inefficient utilization of lignin-derived aromatic compounds. These issues have limited the industrial application of renewable feedstocks in bioprocesses.
“By demonstrating how A. baylyi ADP1 can detoxify inhibitory compounds and convert lignin monomers into valuable products through engineered metabolic pathways, my study provides practical solutions to improve the robustness and efficiency of microbial production systems,” says Liu.
Liu’s findings contribute to the development of more sustainable and economically viable biotechnological processes for replacing fossil-based chemicals with bio-based alternatives.
“This study highlights the versatility of A. baylyi ADP1 as a microbial platform for lignocellulosic biomass valorization. Its ability to detoxify inhibitors, cooperate in microbial consortia, and produce high-value chemicals makes it a promising candidate for future biotechnological applications,” he adds.
Originally from China, Changshuo Liu is currently a doctoral researcher in the Synthetic biology (Synbio) research group at Tampere University.
Public defence on Friday 12 September
The doctoral dissertation of MSc (Tech) Changshuo Liu in the field of biotechnology titled The Multifaceted Role of Acinetobacter baylyi ADP1 in Upgrading Lignocellulose will be publicly examined at the Faculty of Engineering and Natural Sciences at Tampere University at 12 o’clock on Friday 12 September 2025 at Hervanta campus, in Festia building, auditorium Pieni Sali 1 (Korkeakoulunkatu 8, Tampere).
The Opponent will be Professor Mikko Metsä-Ketelä from the University of Turku. The Custos will be Docent Suvi Santala from the Faculty of Engineering and Natural Sciences, Tampere University.