General description

Course organized by TUT, see TUT study guide for up-to-date information.

Learning outcomes

After the course, the student can:
- Identify, list and define common techniques used in genetic engineering and gene expression analysis,
- Interpret data generated from the techniques discussed, classify strengths and weaknesses of the methodologies, summarize results and methods, infer consequences of results, explain procedures.
- Apply the acquired knowledge to better interpret degrees of confidence in experimental results.
- Deconstruct results of gene expression measurements. Compare different methodologies for verifying a hypothesis or measuring a variable.
- Compare and appraise related methods in gene expression studies, and interpret and comment conclusions in such studies,
- Create and develop an experimental procedure using the methods learned, that can address simple questions regarding gene expression.

Contents

- The structure, design and applicability of the bacterial plasmid vector to clone a gene of interest and express protein.
- The introduction of genomic insertion by homologous recombination.
- Studies of bacterial gene expression.
- Studies of variability in bacterial gene expression at the single cell level, with an emphasis on cell-to-cell variation.
- Application and analysis of PCR method.
- The theory and practice of fluorescence microscopy, as used to test for protein expression and localization.

Evaluation

Belongs to following study modules

2018–2019

Teaching

Archived Teaching Schedule. Please refer to current Teaching Shedule.

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