Mass Spectrometry Facility
Tutkimusinfrastruktuuri

Mass Spectrometry Facility

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Mass Spectrometry Facility

Introduction

NanoLC-MSTOF liquid chromatography-mass spectrometry is an analytical technique, which first separates compounds based on their chemical properties according to a gradient flow, and then ionizes the chemical compounds and identifies them based on mass-to-charge ratio and fragmentation patterns.

Our facility has specialized in label-free relative quantitation of proteins from small clinical samples using SWATH-MS method. The facility has broad proteomics capabilities offering analysis for small and large-scale studies, including basic protein identification studies as well as clinical trials/discovery studies. Facility can provide full service with sample preparation, analysis and bioinformatics if needed.

Typical protein discovery study includes SWATH-MS relative quantitation library creation, sample preparation, SWATH-MS analysis, relative quantitation of results and bioinformatics. Typical study using tissue samples or cell lysates with relatively simple sample handling yields 3000-4000 relatively quantified proteins per sample.

Possible samples include e.g.

  • Body fluid (tear, blood, plasma, urine etc.)
  • Tissue (fresh or paraffin-embedded)
  • Cell lysate
  • Cell culture medium
  • Extracellular vesicles
  • Pull-down mixture
  • Protein band from SDS-PAGE gel

We can also provide additional confirmation/validation analyses using MicroLC-MSTrap equipment for known targets.

We have established multiple quantification libraries. Please enquire if we already have library, which suits your needs.

Please fill out our inquiry form (pdf) and we are happy to discuss your needs further!

 

ACKNOWLEDGEMENT

All the users of the Mass Spectrometry Facility services are obligated to acknowledge the facility in publications:

“The authors acknowledge the Tampere Mass Spectrometry Facility for their service.”