Droplet Digital PCR
Tutkimusinfrastruktuuri

Droplet Digital PCR

Tiedekunta tai osaamisyksikkö: Lääketieteen ja terveysteknologian tiedekunta

The facility offers instruments and materials for performing droplet digital PCR. The instruments are available to all research groups and collaborators inside and outside of Tampere University.

Introduction

Droplet digital PCR (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. A sample is fractionated into 20 000 droplets, and PCR amplification of the template molecules occurs in each individual droplet. ddPCR technology uses reagents and workflows similar to quantitative PCR. The massive sample partitioning is a key aspect of the ddPCR technique.

Read more about the technology from the manufacturer’s website: https://www.bio-rad.com/en-fi/applications-technologies/droplet-digital-pcr-ddpcr-technology.

Short ddPCR core facility introduction can be found here: