Smart upgrading of biomass by multispecies approach

Lignocellulose represents the major renewable resource for the future production of oleochemicals and bioenergy compounds by biological systems. Challenges related to the recalcitrance and heterogeneity of the biomass restrict its efficient use. This research proposes a novel approach for comprehensive utilization and valorization of lignocellulosic biomass by a microbial production process based on two different species, an oleaginous yeast and a lignin component-utilizing bacterium. The strains are engineered in terms of improved tolerance against the inhibitory compounds present in lignocellulose hydrolysates and more efficient carbon utilization and production of high-value lipid products. Metabolic engineering strategies and bioprocess engineering are employed to intensify lignocellulose upgrading.