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Mass Spectrometry Facility
Research infrastructure

Mass Spectrometry Facility

Tampere University
Faculty of Medicine and Health Technology
Kauppi Campus
Arvo Ylpön katu 34, 33520 Tampere
 
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MET core facilities and services
Mass Spectrometry Facility

Core facility will cease operations at the end of 2023

Introduction

NanoLC-MSTOF liquid chromatography-mass spectrometry is an analytical technique, which first separates compounds based on their chemical properties according to a gradient flow, and then ionizes the chemical compounds and identifies them based on mass-to-charge ratio and fragmentation patterns.

Our facility has specialized in label-free relative quantitation of proteins from small clinical samples using SWATH-MS method. The facility has broad proteomics capabilities offering analysis for small and large-scale studies, including basic protein identification studies as well as clinical trials/discovery studies. Facility can provide full service with sample preparation, analysis and bioinformatics if needed.

Typical protein discovery study includes SWATH-MS relative quantitation library creation, sample preparation, SWATH-MS analysis, relative quantitation of results and bioinformatics. Typical study using tissue samples or cell lysates with relatively simple sample handling yields 3000-4000 relatively quantified proteins per sample.

Possible samples include e.g.

  • Body fluid (tear, blood, plasma, urine etc.)
  • Tissue (fresh or paraffin-embedded)
  • Cell lysate
  • Cell culture medium
  • Extracellular vesicles
  • Pull-down mixture
  • Protein band from SDS-PAGE gel

We have established multiple quantification libraries. Please enquire if we already have library, which suits your needs.

Please fill out our inquiry form (pdf) and we are happy to discuss your needs further!

MSF-inquiry-form

ACKNOWLEDGEMENT

All the users of the Mass Spectrometry Facility services are obligated to acknowledge the facility in publications:

“The authors acknowledge the Tampere Mass Spectrometry Facility for their service.”

MET Core Facilities and Services

Tampere University and Tampere University of Applied Sciences (TAMK) constitute the Tampere Universities community. Our areas of priority in research and education are technology, health and society.

Tampere University: +358 (0)294 5211
Tampere University of Applied Sciences : +358 (0)294 5222